Methods and compositions for plant pest control
First Claim
1. A method for producing a barley plant exhibiting an improvement in fungal disease resistance comprising topically applying to a barley plant surface a composition that comprises:
- a. at least one dsRNA of 24 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 24 contiguous nucleotides that are identical or fully complementary to SEQ ID NO;
184, wherein said dsRNA is not operably linked to a promoter or to a viral vector and wherein said dsRNA does not become integrated into the plant chromosome; and
b. a transfer agent comprising an organosilicone preparation that conditions the barley plant surface to permeation by the dsRNA molecules into plant cells, wherein said plant exhibits an improvement in fungal disease resistance in comparison to a control barley plant that has not been treated with a composition comprising the dsRNA and a transfer agent, wherein the fungal disease resistance results from suppression of a barley Mildew Resistance Locus O (MLO) gene.
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Abstract
The present invention provides methods and compositions to improve fungal disease resistance and/or nematode resistance in various crop plants. The present invention also provides for combinations of compositions and methods to improve fungal disease resistance and/or nematode resistance in various crop plants. Powdery mildews are fungal diseases that affect a wide range of plants including cereals, grasses, vegetables, ornamentals, weeds, shrubs, fruit trees, broad-leaved shade and forest trees, that is caused by different species of fungi in the order Erysiphales.
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Citations
9 Claims
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1. A method for producing a barley plant exhibiting an improvement in fungal disease resistance comprising topically applying to a barley plant surface a composition that comprises:
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a. at least one dsRNA of 24 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 24 contiguous nucleotides that are identical or fully complementary to SEQ ID NO;
184, wherein said dsRNA is not operably linked to a promoter or to a viral vector and wherein said dsRNA does not become integrated into the plant chromosome; andb. a transfer agent comprising an organosilicone preparation that conditions the barley plant surface to permeation by the dsRNA molecules into plant cells, wherein said plant exhibits an improvement in fungal disease resistance in comparison to a control barley plant that has not been treated with a composition comprising the dsRNA and a transfer agent, wherein the fungal disease resistance results from suppression of a barley Mildew Resistance Locus O (MLO) gene. - View Dependent Claims (2, 3, 4, 5)
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6. A composition comprising at least one dsRNA of 24 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 24 nucleotides that are identical or fully complementary to SEQ ID NO:
- 184, wherein said dsRNA is not operably linked to a promoter or a viral vector; and
, b) a transfer agent comprising an organosilicone preparation that conditions a plant surface to permeation by the dsRNA molecules into plant cells. - View Dependent Claims (7, 8, 9)
- 184, wherein said dsRNA is not operably linked to a promoter or a viral vector; and
Specification