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Methods and compositions for plant pest control

  • US 10,077,451 B2
  • Filed: 10/18/2013
  • Issued: 09/18/2018
  • Est. Priority Date: 10/18/2012
  • Status: Active Grant
First Claim
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1. A method for producing a barley plant exhibiting an improvement in fungal disease resistance comprising topically applying to a barley plant surface a composition that comprises:

  • a. at least one dsRNA of 24 to about 95 nucleotides in length, wherein the dsRNA comprises a segment of 24 contiguous nucleotides that are identical or fully complementary to SEQ ID NO;

    184, wherein said dsRNA is not operably linked to a promoter or to a viral vector and wherein said dsRNA does not become integrated into the plant chromosome; and

    b. a transfer agent comprising an organosilicone preparation that conditions the barley plant surface to permeation by the dsRNA molecules into plant cells, wherein said plant exhibits an improvement in fungal disease resistance in comparison to a control barley plant that has not been treated with a composition comprising the dsRNA and a transfer agent, wherein the fungal disease resistance results from suppression of a barley Mildew Resistance Locus O (MLO) gene.

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