Volume sensing in fluidic cartridge

• US 10,077,999 B2
• Filed: 07/13/2016
• Issued: 09/18/2018
• Est. Priority Date: 07/14/2015
• Status: Active Grant

• Current Assignee: Cytochip Inc.
• Original Assignee: Cytochip Inc.
• Inventors: Shi, Wendian, Ding, Yuzhe
• Primary Examiner(s): Shah, Manish S
• Assistant Examiner(s): Plumb, Nigel
• Application Number:
  US15/209,226
• Publication Number:
  US 20170016753A1
• Time in Patent Office: 797 Days
• Field of Search: 73149, 73426, 73 6161, 73 6152, 73 6143, 73 5301, 73 6141, 73 136, 73 101
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01F 1/002 wherein the flow is in an open channel
• CPC Class Codes: G01F 1/7086 using optical detecting arrangements
• CPC Class Codes: G01F 1/74 Devices for measuring flow of a fluid or flow of a fluent solid material in suspension in another fluid
• CPC Class Codes: G01F 22/00 Methods or apparatus for measuring volume of fluids or fluent solid material, not otherwise provided for
• CPC Class Codes: G01F 23/265 for discrete levels

Abstract

Methods and apparatuses for measuring parameters based on the presence or absence of fluid at a plurality of fluid sensing zones in a disposable cassette are described herein. In an embodiment, a fluidic device includes a disposable cartridge including a fluid channel, an instrument configured to receive the disposable cartridge, the instrument including at least one sensor having a plurality of fluid sensing zones spaced along the fluid channel when the disposable cartridge is received by the disposable cartridge, each fluid sensing zone configured to determine a presence or absence of fluid at a respective portion of the fluid channel, and a control unit configured to determine at least one of a fluid volume, a volume displacement, a flow rate, a flow velocity or a volume ratio of gas bubbles of the fluid based on the presence or absence of the fluid at each of the fluid sensing zones.

Claims

1. A fluidic device comprising:

a disposable cartridge including a fluid channel configured to receive a fluid flow;

an instrument configured to receive the disposable cartridge, the instrument including at least one sensor having a plurality of fluid sensing zones spaced along the fluid channel when the disposable cartridge is received by the instrument, each fluid sensing zone configured to determine a presence or absence of fluid at a respective portion of the fluid channel; and

a control unit configured to (i) receive a signal indicative of the presence or absence of the fluid at each of the fluid sensing zones, and (ii) determine a volume of a fluid plug or a volume ratio of gas bubbles in a fluid based on the presence or absence of the fluid at each of the fluid sensing zones.

2. The fluidic device of claim 1, wherein the plurality of fluid sensing zones are spaced along a length of the fluid channel when the disposable cartridge is received by the instrument.

3. The fluidic device of claim 1, wherein the plurality of fluid sensing zones are spaced along a width of the fluid channel when the disposable cartridge is received by the instrument.

4. The fluidic device of claim 1, wherein the plurality of fluid sensing zones are each located on separate sensors configured to measure the presence or absence of fluid at the respective portion of the fluid channel along the length of the fluid channel.

5. The fluidic device of claim 1, wherein the plurality of fluid sensing zones are each located on the same sensor, wherein the fluid channel has a winding shape through the disposable cartridge, and wherein the plurality of fluid sensing zones are aligned with sections of the fluid channel that converge into a centralized portion of the sensor.

6. The fluidic device of claim 1, wherein the fluid channel has a known cross-sectional area, and wherein the control unit uses the known cross-sectional area of the fluid channel to determine at least one of a volume displacement, a flow rate or a flow velocity of the fluid based on the presence or absence of the fluid at each of the fluid sensing zones.

7. A fluidic device comprising:

a disposable cartridge including a fluid channel configured to receive a fluid flow;

an instrument configured to receive the disposable cartridge, the instrument including at least one sensor having a plurality of fluid sensing zones spaced along a length of the fluid channel when the disposable cartridge is received by the instrument, each sensor including a light emitter and a light receiver; and

a control unit configured to determine a volume displacement of a fluid flow or a volume of a fluid plug in the fluid channel based on a changed level of light transmission in a first sensing zone and a changed level of light transmission in a second sensing zone, wherein the level of light transmission is changed by the amount of light scattered with presence or absence of fluid in the fluid sensing zone.

8. The fluidic device of claim 7, wherein the controller is configured to use a surface roughness of at least a portion of the fluid channel to determine the presence or absence of fluid at each of the fluid sensing zones, wherein the surface roughness changes the amount of light scattered in each sensor when there is presence or absence of fluid.

9. The fluidic device of claim 7, wherein the controller is configured to use a refractive index of a material forming at least a portion of the fluid channel to determine the presence or absence of fluid at each of the fluid sensing zones, wherein the difference between the refractive index of the material and the refractive index of fluid in the channel changes the amount of light scattered in each sensor.

10. The fluidic device of claim 7, wherein the controller is configured to determine at least one of a flow velocity or a volume ratio of gas bubbles of the fluid based on detecting the fluid flow entering each of the fluid sensing zones.

23. The fluidic device of claim 7, wherein the fluid channel overlapping with the fluid sensing zones is configured to have a surface roughness that scatters at least a portion of the light transmission from the respective emitter to the respective receiver, and the control unit is configured to determine a fluid entering the fluid sensing zones by detecting an increased level of the light transmission.

24. The fluidic device of claim 23, wherein the surface roughness of the fluid channel overlapping with the sensing zones is in the range of about 0.1 nm to 10 nm, 10 nm to 1 um, 1 um to 10 um or 10 um to 100 um.

25. The fluidic device of claim 7, wherein the instrument comprises two or more sensors, and the controller is configured to determine the volume displacement of the fluid flow or the volume of the fluid plug in the fluid channel by detecting a changed level of light transmission in a first sensor and a changed level of light transmission in a second sensor.

26. The fluidic device of claim 7, wherein the at least one sensor comprises a light masking aperture.

27. The fluidic device of claim 9, wherein a material forming at least a portion of the fluid channel is configured to have a refractive index of about 1.3 to 1.7.

11. A method of configuring a disposable cartridge comprising:

designing a disposable cartridge to have a fluid channel configured to receive a particular fluid sample;

configuring the fluid channel to align with a plurality of fluid sensing zones corresponding to at least one light emitter and at least one light receiver of a reader instrument when the disposable cartridge is received in the instrument for measurements; and

determining a fluid volume displacement, a volume of a fluid plug or a volume ratio of gas bubbles in a fluid based on the presence or absence of the fluid at each of the fluid sensing zones.

12. The method of claim 11, which includes programming the reader instrument to determine the presence or absence of fluid in the fluid channel of the disposable cartridge based on the light transmission from the respective emitter to the respective receiver, wherein the level of light transmission is changed by the amount of light scattered when there is a presence or absence of fluid in the channel.

13. The method of claim 11, which includes configuring the portion of the fluid channel to align with a plurality of light emitters and light receivers along the width of the fluid channel.

14. The method of claim 11, which includes configuring the portion of the fluid channel to align with a plurality of light emitters and light receivers along the length of the fluid channel.

15. The method of claim 14, which includes allowing for an alignment tolerance when aligning the plurality of light emitters and light receivers with the fluid channel in the cartridge by fixing the distance between the plurality of light emitters and light receivers before alignment.

16. A fluidic device comprising:

a disposable cartridge including a fluid channel configured to receive a fluid flow;

an instrument configured to receive the disposable cartridge, the instrument including at least one sensor having a plurality of fluid sensing zones spaced along the fluid channel when the disposable cartridge is received by the instrument, each sensor comprising a light emitter, a light receiver and a plurality of light masking apertures defining multiple fluid sensing zones; and

a control unit configured to (i) receive a signal indicative of the presence or absence of the fluid at each of the fluid sensing zones, wherein the signal indicates the level of the light transmitted from the respective light emitter to the respective light receiver at the fluid sensing zones, and (ii) analyze the received signal to determine a volume of a fluid plug or a volume ratio of gas bubbles in a fluid based on a presence or absence of the fluid at each of the fluid sensing zones.

17. The fluidic device of claim 16, wherein the plurality of fluid sensing zones are spaced along a length of the fluid channel when the disposable cartridge is received by the instrument.

18. The fluidic device of claim 16, wherein the plurality of fluid sensing zones are spaced along a width of the fluid channel when the disposable cartridge is received by the instrument.

19. The fluidic device of claim 16, wherein the plurality of fluid sensing zones are each located on separate sensors configured to measure the presence or absence of fluid at the respective portion of the fluid channel along the length of the fluid channel.

20. The fluidic device of claim 16, wherein the instrument comprises at least one sensor that has two or more sensing zones.

21. The fluidic device of claim 16, wherein at least one sensor comprises a light emitter, a light receiver and two or more light masking apertures defining two or more of the fluid sensing zones, and wherein the controller unit is configured to detect three different levels of signal from the at least one sensor for the following:

(i) presence of fluid in one fluid sensing zone;

(ii) presence of fluid in two fluid sensing zones; and

(ii) absence of fluid in the fluid sensing zones.

22. The fluidic device of claim 16, wherein each light masking aperture is initially separated from the disposable cartridge and overlaps with the fluid channel of the disposable cartridge after the disposable cartridge is received by the instrument.