Spraying system and methods of use thereof

• US 10,081,026 B2
• Filed: 12/12/2016
• Issued: 09/25/2018
• Est. Priority Date: 12/07/2010
• Status: Active Grant

• Current Assignee: University of Florida Research Foundation, Incorporated (State University System of Florida)
• Original Assignee: University of Florida Research Foundation, Incorporated (State University System of Florida)
• Inventors: Garrett, Timothy James, Mounfield, III, William Pratt
• Primary Examiner(s): Leong, Nathan T
• Application Number:
  US15/375,799
• Publication Number:
  US 20170087572A1
• Time in Patent Office: 652 Days
• Field of Search: None
• CPC Class Codes: B05B 7/0416 with arrangements for mixing one gas and one liquid
• CPC Class Codes: B05B 7/0441 with one inner conduit of liquid surrounded by an external conduit of gas upstream the mixing chamber
• CPC Class Codes: B05B 7/12 designed to control volume of flow, e.g. with adjustable passages
• CPC Class Codes: B05B 7/1209 the controlling means for each liquid or other fluent material being manual and interdependent
• CPC Class Codes: B05D 1/02 performed by spraying
• CPC Class Codes: H01J 49/0418 for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates

Abstract

Embodiments of the present disclosure provide for spraying systems, methods of disposing a layer of matrix onto a sample, and the like.

Claims

1. A method of disposing a layer of a matrix on a sample, comprising:

providing at least one sample;

providing a spraying system comprising;

a liquid system for providing a liquid to a nebulizer;

a gas system for providing a gas to the nebulizer, wherein the nebulizer is configured to produce a nebulized liquid;

a control system for controlling the introduction of the liquid, the gas, or the liquid and gas, to the nebulizer; and

a sample system, wherein the sample system includes an area for the at least one sample to be disposed,

wherein the nebulizer directs the liquid onto the area where the sample is to be disposed via a nozzle; and

disposing a layer of a matrix uniformly onto a portion of the at least one sample in about 2 to 30 seconds, wherein the layer of matrix is about 100 nm to 500 μ

m thick.

2. The method of claim 1, wherein a sample is about 10 to 20 μ

m thick.

3. The method of claim 1, wherein the portion of a sample includes the entire sample.

4. The method of claim 1, wherein disposing is performed in less than 20 seconds.

5. The method of claim 1, further comprising repeating the disposing step one or more times to produce the layer of matrix.

6. The method of claim 1, wherein disposing includes nebulizing a liquid that includes the matrix and wherein the nebulized liquid is directed towards the at least one sample.

7. The method of claim 6, wherein the liquid includes a solvent, wherein the solvent is selected from the group consisting of:

chloroform, methanol, ethanol, isopropanol, water, t-butyl-methyl ether, an acid except hydrochloric acid, a base, and a combination thereof.

8. The method of claim 6, wherein the matrix is selected from the group consisting of:

2,5-dihydroxybenzoic acid, alpha-cyano-4-hydroxycinnamic acid, sinipinic acid, trihydroxyacetophenone, dihydroxyacetophenone, and a combination thereof.

9. The method of claim 6, wherein nebulization includes a gas, wherein the gas is selected from the group consisting of:

a noble gas, oxygen, helium, water vapor, and a combination thereof.

10. A method of disposing a layer of a matrix on a sample, comprising:

providing at least one sample;

providing a spraying system comprising;

a liquid system for providing a single liquid to a nebulizer, wherein the liquid system includes a liquid delivery system selected from the group consisting of;

a gravity liquid delivery system and a siphon liquid delivery system;

a gas system for providing a gas to the nebulizer, wherein the nebulizer is configured to produce a nebulized liquid from the liquid from the liquid system;

a control system for controlling introduction of the liquid, the gas, or the liquid and gas, to the nebulizer; and

a sample system, wherein the sample system includes an area for the at least one sample to be disposed;

wherein the nebulizer directs the nebulized liquid onto the area where the sample is to be disposed via a nozzle of the nebulizer; and

wherein the nebulizer and the area where the at least one sample is to be disposed are disposed in a chamber; and

disposing a layer of a matrix uniformly onto a portion of the at least one sample in about 2 to 30 seconds, wherein the layer of matrix is about 100 nm to 500 μ

m thick.

11. The method of claim 10, wherein the nebulizer mixes the gas and liquid within the nebulizer.

12. The method of claim 10, wherein the control system is selected from a manual system, an automated system, or a combination thereof.

13. The method of claim 10, wherein the nebulizer is positioned above a horizontal, vertical, or inverted, position relative to the area where the sample is to be disposed.

14. The method of claim 10, wherein the chamber is heated and the nebulizer is heated.

15. The method of claim 10, wherein the nebulizer is about 0.01 to 60 inches from the area where the sample is to be disposed.

16. The method of claim 10, wherein the liquid system is adapted to heat the liquid.

17. The method of claim 10, wherein the gas system is adapted to heat the gas.

18. The method of claim 10, wherein sample system is adapted to move up and down along an y-axis and from side to side along an x-axis and a z-axis.

19. The method of claim 10, wherein the sample system includes an automated platform to position samples in sequence in the area where the sample is to be disposed.

20. The method of claim 10, wherein the chamber is a humidity controlled chamber.

21. The method of claim 10, wherein the liquid system is adapted to cool the liquid.