System and method for electrospun biodegradable scaffold for bone repair

• US 10,081,794 B2
• Filed: 04/13/2012
• Issued: 09/25/2018
• Est. Priority Date: 04/13/2011
• Status: Active Grant

• Current Assignee: New Jersey Institute of Technology
• Original Assignee: New Jersey Institute of Technology
• Inventors: Arinzeh, Treena Lynne, Briggs, Tamunotonye
• Primary Examiner(s): Yamasaki, Robert J
• Application Number:
  US14/111,638
• Publication Number:
  US 20150087062A1
• Time in Patent Office: 2,356 Days
• Field of Search: None
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2300/602 Type of release, e.g. controlled, sustained, slow
• CPC Class Codes: A61L 2300/802 Additives, excipients, e.g. cyclodextrins, fatty acids, surfactants
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/46 with phosphorus-containing inorganic fillers
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 71/02 Polyalkylene oxides
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 2537/00 Supports and/or coatings for cell culture characterised by physical or chemical treatment
• CPC Class Codes: C12N 5/0654 Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth

Abstract

This invention relates a structure and system for growth factor incorporation which can improve the osteogenic differentiation of hMSCs, for potential bone regeneration and bone growth applications or used alone for bone repair or growth applications. The system comprises a biodegradable polyester, a hydrophilic polymer, a growth factor and optionally a bioceramic.

Claims

1. An electrospun composite scaffold comprising a polyester, a hydrophilic polymer, a bioceramic that includes phosphate ions, a cationic surfactant that complexes with the phosphate ions of the bioceramic, and a growth factor or a protein, wherein complexing of the cationic surfactant with the phosphate ions of the bioceramic decreases electrostatic attraction of the growth factor or the protein to the bioceramic, thereby enhancing release of the growth factor or the protein from the scaffold relative to release from the same scaffold without a cationic surfactant.

2. The electrospun composite scaffold of claim 1, wherein the polyester is selected from the group consisting of polylactic acid, polyglycolic acid, polylactic co-glycolic acid copolymers and polycaprolactone.

3. The electrospun composite scaffold of claim 1, wherein the hydrophilic polymer is selected from the group consisting of polyethylene oxide, polyethylene glycol, polyvinyl alcohol, glycosaminoglycans, chitosan, sulfated dextran, sulfated cellulose, and heparin sulfate.

4. The electrospun composite scaffold of claim 1, wherein the growth factor is selected from the group consisting of Recombinant human Platelet Derived Growth Factor-BB (PDGF-BB), vascular endothelial factor (VEGF), transforming growth factor-beta (tgf-BETA) and Bone Morphogenetic Protein (BMP).

5. The electrospun composite scaffold of claim 1, wherein the protein is lysozyme.

6. The electrospun composite scaffold of claim 1, wherein the bioceramic is selected from the group consisting of hydroxyapatite, tricalcium phosphate, and biphasic calcium phosphate.

7. The electrospun composite scaffold of claim 1, wherein the cationic surfactant is cetyl trimethylammonium bromide (CTAB).