Mapping cytosine modifications
First Claim
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1. A method for selectively altering any modified cytosine in a nucleic acid that is hydroxymethylcytosine (hmC) or methylcytosine (mC), comprising:
- (a) reacting the nucleic acid with UDP-glucosamine in the presence of a glycosyltransferase to convert hmC in the nucleic acid to glucosaminylated cytosine, and(b) reacting the nucleic acid with an oxidizing agent and UDP-glucose to convert mC to glucosylated methylcytosine wherein the oxidizing agent is methyl cytosine oxygenase or KRuO4.
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Abstract
Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.
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Citations
9 Claims
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1. A method for selectively altering any modified cytosine in a nucleic acid that is hydroxymethylcytosine (hmC) or methylcytosine (mC), comprising:
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(a) reacting the nucleic acid with UDP-glucosamine in the presence of a glycosyltransferase to convert hmC in the nucleic acid to glucosaminylated cytosine, and (b) reacting the nucleic acid with an oxidizing agent and UDP-glucose to convert mC to glucosylated methylcytosine wherein the oxidizing agent is methyl cytosine oxygenase or KRuO4. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification