Device for preparation and analysis of nucleic acids

• US 10,087,440 B2
• Filed: 05/07/2014
• Issued: 10/02/2018
• Est. Priority Date: 05/07/2013
• Status: Active Grant

• Current Assignee: Revvity Health Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Micronics Inc. (Sony Group Corp.)
• Inventors: Lofquist, Alan K., Battrell, C. Frederick, Bouzek, Heather K.
• Primary Examiner(s): Jarrett, Lore R
• Application Number:
  US14/889,374
• Publication Number:
  US 20160090588A1
• Time in Patent Office: 1,609 Days
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: C12N 15/1017 by filtration, e.g. using filters, frits, membranes
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence

Abstract

An integrated “lab-on-a-chip” microfluidic device performs nucleic acid sample preparation and diagnostic analysis from test samples containing cells and/or particles. The device analyzes DNA or RNA targets, or both, from a common test sample. Dried and/or liquid reagents necessary for nucleic acid sample preparation and analysis are contained on the device, such that the device only requires addition of test sample. Clay mineral and alkaline buffer reagents are employed for overcoming the problems of nucleic acid degradation and contamination during sample preparation. The device may include a composite filter to separate plasma or serum from other blood constituents when the test sample is a blood product. The microfluidic device utilizes a plurality of microfluidic channels, inlets, valves, membranes, pumps, and other elements arranged in various configurations to manipulate the flow of the liquid sample, in particular, in order to prepare nucleic acids and perform further diagnostic analysis.

Claims

1. A microfluidic device comprising:

a microfluidic channel having a first end and a second end;

a sample inlet fluidly connected to the first end of the microfluidic channel and configured for receiving a test sample;

a clay treatment chamber fluidly connected to said microfluidic channel, wherein said clay treatment chamber contains a clay mineral reagent;

a sample lysis chamber fluidly connected to said clay treatment chamber, wherein said sample lysis chamber contains an alkaline solution;

one or more sample nucleic acid amplification or detection wells, or combinations thereof, fluidly connected to said sample lysis chamber; and

and one or more sample outlets,

wherein said clay treatment chamber is upstream of said sample lysis chamber and said one or more sample nucleic acid amplification or detection wells, and wherein said device is configured such that said test sample exits said clay treatment chamber and enters said sample lysis chamber, thereby contacting said test sample with said alkaline solution to release target nucleic acids.

2. The microfluidic device of claim 1, wherein the clay mineral comprises a kaolinite, smectite, or illite clay mineral.

3. The microfluidic device of claim 1, wherein the clay mineral comprises talc.

4. The microfluidic device of claim 1, wherein the clay mineral comprises halloysite.

5. The microfluidic device of claim 1, wherein the clay mineral comprises bentonite.

6. The microfluidic device of claim 1, wherein the clay mineral comprises a synthetic clay mineral.

7. The microfluidic device of claim 6, wherein the synthetic clay mineral is laponite.

8. The microfluidic device of claim 1, wherein the alkaline solution comprises KOH, NaOH, or LiOH.

9. The microfluidic device of claim 8, wherein the alkaline solution comprises KOH.

10. The microfluidic device of claim 1, further comprising a neutralization chamber downstream of the lysis chamber, wherein the neutralization chamber contains an acidic reagent.

11. The microfluidic device of claim 10, wherein the acidic solution comprises HCl, C₂H₄O₂,or H₂SO₄.

12. The microfluidic device of claim 1, wherein the test sample comprises one or more infectious agents.

13. The microfluidic device of claim 12, wherein the one or more infectious agents are viral agents.

14. The microfluidic device of claim 1, wherein the test sample comprises at least two viral agents.

15. The microfluidic device of claim 1, wherein the test sample comprises a DNA virus and an RNA virus.

16. The microfluidic device of claim 15, wherein the DNA virus is HBV.

17. The microfluidic device of claim 15, wherein the RNA virus is HCV or HIV.

18. The microfluidic device of claim 1, wherein the test sample is selected from the group consisting of blood, plasma, serum, urine, saliva, sputum, respiratory lavage, tears, and tissue swabs.

19. The microfluidic device of claim 1, wherein the test sample is selected from the group consisting of blood, plasma, and serum.

20. The microfluidic device of claim 1, further comprising an on-device pump fluidly connected to the second end of the microfluidic channel.

21. The microfluidic device of claim 1, further a comprising a composite membrane interposed between the sample inlet and the first end of the microfluidic channel, wherein the composite membrane is capable of removing selected particles from blood.

22. The microfluidic device of claim 21, wherein the composite membrane comprises a material that activates blood coagulation.

23. The microfluidic device of claim 22, wherein the composite membrane comprises a glass filter.