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Device for preparation and analysis of nucleic acids

  • US 10,087,440 B2
  • Filed: 05/07/2014
  • Issued: 10/02/2018
  • Est. Priority Date: 05/07/2013
  • Status: Active Grant
First Claim
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1. A microfluidic device comprising:

  • a microfluidic channel having a first end and a second end;

    a sample inlet fluidly connected to the first end of the microfluidic channel and configured for receiving a test sample;

    a clay treatment chamber fluidly connected to said microfluidic channel, wherein said clay treatment chamber contains a clay mineral reagent;

    a sample lysis chamber fluidly connected to said clay treatment chamber, wherein said sample lysis chamber contains an alkaline solution;

    one or more sample nucleic acid amplification or detection wells, or combinations thereof, fluidly connected to said sample lysis chamber; and

    and one or more sample outlets,wherein said clay treatment chamber is upstream of said sample lysis chamber and said one or more sample nucleic acid amplification or detection wells, and wherein said device is configured such that said test sample exits said clay treatment chamber and enters said sample lysis chamber, thereby contacting said test sample with said alkaline solution to release target nucleic acids.

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