Referencing in multi-acquisition slide imaging
First Claim
1. A computer-implemented method for registering images, comprising:
- acquiring a first set of images of a sample on a slide positioned on a stage, wherein each image is taken at a different field of view;
generating a global transformation matrix by registering the images of the first set to one another to form a composite image;
acquiring a subset of images of the sample on the slide after the slide is removed from and replaced on the stage, wherein the subset of images is less than the number of images in the first set;
registering each image of the subset with a corresponding image of the first set of images to determine a translation, a rotation, and a scale factor;
acquiring a second set of images of the sample at the same respective fields of view used to acquire the first set of images;
rotating and scaling each image of the second set of images using the rotation and the scale factor determined for the subset of images; and
registering the rotated and scaled images of the second set to the corresponding images of the first set.
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Abstract
Referencing of image acquired in multiple rounds of imaging is disclosed. In certain implementations, a baseline round of images are acquired and registered to one another to establish a global transformation matrix. In a subsequent round of image acquisition, a limited number of field of view images are initially acquired and registered to the corresponding baseline images to solve for translation, rotation, and scale. The full set of baseline images is then acquired for the subsequent round and each image is pre-rotated and pre-scaled based on the transform determined for the subset of images. The pre-rotated, pre-scaled images are then registered using a translation-only transform.
50 Citations
20 Claims
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1. A computer-implemented method for registering images, comprising:
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acquiring a first set of images of a sample on a slide positioned on a stage, wherein each image is taken at a different field of view; generating a global transformation matrix by registering the images of the first set to one another to form a composite image; acquiring a subset of images of the sample on the slide after the slide is removed from and replaced on the stage, wherein the subset of images is less than the number of images in the first set; registering each image of the subset with a corresponding image of the first set of images to determine a translation, a rotation, and a scale factor; acquiring a second set of images of the sample at the same respective fields of view used to acquire the first set of images; rotating and scaling each image of the second set of images using the rotation and the scale factor determined for the subset of images; and registering the rotated and scaled images of the second set to the corresponding images of the first set. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. An image analysis system, comprising:
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a memory storing one or more routines; and a processing component configured to execute the one or more routines stored in the memory, wherein the one or more routines, when executed by the processing component, cause acts to be performed comprising; performing an alignment operation between a limited set of images acquired of a sample and a previously acquired full set of images of the sample wherein the limited set of images comprise fewer images than the full set of images, wherein the alignment operation generates at least a rotation relating the limited set of images to corresponding images of the full set of images; rotating a subsequently acquired full set of images by the rotation; and translating the rotated images of the subsequently acquired full set of images to register the rotated images with corresponding images of the previously acquired full set of images. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A digital microscopy imaging system, comprising:
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a stage configured to hold a slide; an objective lens and image sensor configured to acquire images of a portion of the slide, when present; a position controller configured to move one or both of the objective lens and the stage relative to one another in accordance with an imaging protocol; a controller configured to control operation of one or both of the position controller and the image sensor; a memory storing one or more routines; and a processing component configured to execute the one or more routines stored in the memory, wherein the one or more routines, when executed by the processing component, cause acts to be performed comprising; acquiring a first full set of images of at least a portion of the slide, wherein each image overlaps with at least one adjacent image; acquiring a second full set of images of at least the portion of the slide after the slide is removed from and replaced on the stage; rotating the images of the second full set of images by a predetermined angle to generate a set of pre-rotated images; and translating the pre-rotated images to register the pre-rotated images to corresponding images of the first full set of images. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification