Selective glucagon-like-peptide-2 (GLP-2) analogues
First Claim
1. A glucagon-like peptide 2 (GLP-2) analogue represented by general Formula II:
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R1—
Z1-His-X2-X3-Gly-X5-X6-X7-X8-X9-X10-X11-X12-X13-X14-X15-X16-X17-Ala-X19-X20-X21-Phe-Ile-X24-Trp-Leu-Ile-X28-Thr-Lys-X31-X32-X33-Z2—
R2
(SEQ ID NO;
4)wherein;
R1 is hydrogen, C1-4 alkyl, methyl, acetyl, formyl, benzoyl or trifluoroacetyl;
X2 is Gly;
X3 is Glu or Asp;
X5 is Ser or Thr;
X6 is Phe;
X7 is Ser or Thr;
X8 is Asp;
X9 is Glu;
X10 is Leu;
X11 is Asn, Ala, Glu, or Ser;
X12 is Thr;
X13 is lie;
X14 is Leu;
X15 is Asp or Glu;
X16 is Ala, Glu, or Ser;
X17 is Leu;
X19 is Ala;
X20 is Ala, Glu, or Ser;
X21 is Asp;
X24 is Ala or Ser;
X28 is Ala, Glu, or Ser;
X31 is lie;
X32 is Thr;
X33 is Asp;
R2 is NH2 or OH;
Z1 and Z2 are independently absent or a peptide sequence of 1-10 amino acid units selected from the group consisting of Ala, Leu, Ser, Thr, Tyr, Asn, Gln, Asp, Glu, Lys, Arg, His, Met and Orn;
or a pharmaceutically acceptable salt thereof.
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Accused Products
Abstract
GLP-2 analogs are disclosed which comprise one of more substitutions as compared to h[Gly2]GLP-2 and which may have the property of an increased small intestine/colon and stomach/colon selectivity. More particularly, preferred GLP-2 analogs disclosed herein comprise substitutions at one or more of positions 11, 16, 20, 24 and/or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 and one or more of positions 3, 5, 7, and 10, and/or a deletion of one or more of amino acids 31 to 33 and/or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogs are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy.
53 Citations
17 Claims
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1. A glucagon-like peptide 2 (GLP-2) analogue represented by general Formula II:
-
R1—
Z1-His-X2-X3-Gly-X5-X6-X7-X8-X9-X10-X11-X12-X13-X14-X15-X16-X17-Ala-X19-X20-X21-Phe-Ile-X24-Trp-Leu-Ile-X28-Thr-Lys-X31-X32-X33-Z2—
R2
(SEQ ID NO;
4)wherein; R1 is hydrogen, C1-4 alkyl, methyl, acetyl, formyl, benzoyl or trifluoroacetyl; X2 is Gly; X3 is Glu or Asp; X5 is Ser or Thr; X6 is Phe; X7 is Ser or Thr; X8 is Asp; X9 is Glu; X10 is Leu; X11 is Asn, Ala, Glu, or Ser; X12 is Thr; X13 is lie; X14 is Leu; X15 is Asp or Glu; X16 is Ala, Glu, or Ser; X17 is Leu; X19 is Ala; X20 is Ala, Glu, or Ser; X21 is Asp; X24 is Ala or Ser; X28 is Ala, Glu, or Ser; X31 is lie; X32 is Thr; X33 is Asp; R2 is NH2 or OH; Z1 and Z2 are independently absent or a peptide sequence of 1-10 amino acid units selected from the group consisting of Ala, Leu, Ser, Thr, Tyr, Asn, Gln, Asp, Glu, Lys, Arg, His, Met and Orn; or a pharmaceutically acceptable salt thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification