Laminous vascular constructs combining cell sheet engineering and electrospinning technologies
First Claim
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1. A tissue engineered vascular construct, comprising:
- an electrospun biocompatible matrix in a tubular configuration; and
a population of smooth muscle cells pre-fabricated as at least one smooth muscle cell sheet, the sheet comprised of smooth muscle cells grown to confluence on a substrate and then detached from said substrate as an intact sheet,wherein the intact sheet is wrapped around the outside of the matrix to form a vascular construct.
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Abstract
Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.
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Citations
27 Claims
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1. A tissue engineered vascular construct, comprising:
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an electrospun biocompatible matrix in a tubular configuration; and a population of smooth muscle cells pre-fabricated as at least one smooth muscle cell sheet, the sheet comprised of smooth muscle cells grown to confluence on a substrate and then detached from said substrate as an intact sheet, wherein the intact sheet is wrapped around the outside of the matrix to form a vascular construct. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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11. A tissue engineered vascular construct, comprising:
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an electrospun biocompatible matrix in a tubular configuration comprising at least one natural component and at least one synthetic polymer component, wherein the natural component comprises collagen and wherein the synthetic polymer component comprises poly(ε
-caprolactone) (PCL); anda population of smooth muscle cells pre-fabricated as at least one smooth muscle cell sheet, the sheet comprised of smooth muscle cells grown to confluence on a substrate and then detached from said substrate as an intact sheet, wherein the intact sheet is wrapped around the outside of the matrix to form a vascular construct. - View Dependent Claims (12)
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Specification