Controlling the activity of growth factors, particularly TGF-β, in vivo
First Claim
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1. A method of releasing active TGF-β
- from a latent TGF-β
complex in which it is sequestered, comprising;
a. conjugating the latent TGF-β
complex to a magnetic nanoparticle; and
b. exposing the resulting conjugate to a radiofrequency magnetic field between approximately 100 kHz and 1 MHz, wherein the magnetic field heats the magnetic nanoparticle sufficient to release active TGF-β
from the latent TGF-β
complex.
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Abstract
TGF-β growth factor and its latent complex are conjugated to magnetic micro- or nanoparticles and to magnetic micro- or nanodiscs. By exposing the resulting conjugates to magnetic fields, the TGF-β growth factor can be released from its latent complex in vivo, potentially making it useful in tissue engineering and regenerative medicine. And by exposing a conjugate of TGF-β growth factor and a magnetic particle to a sufficiently strong, radiofrequency magnetic field, the TGF-β growth factor can be denatured and thereby deactivated, potentially making it possible to avoid triggering tumorigenesis, atherosclerosis, fibrotic disease, and cancer.
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Citations
9 Claims
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1. A method of releasing active TGF-β
- from a latent TGF-β
complex in which it is sequestered, comprising;a. conjugating the latent TGF-β
complex to a magnetic nanoparticle; andb. exposing the resulting conjugate to a radiofrequency magnetic field between approximately 100 kHz and 1 MHz, wherein the magnetic field heats the magnetic nanoparticle sufficient to release active TGF-β
from the latent TGF-β
complex. - View Dependent Claims (2, 3, 4)
- from a latent TGF-β
-
5. A method of releasing active TGF-β
- from a latent TGF-β
complex in which it is sequestered, comprising;a. conjugating the latent TGF-β
complex to a magnetic particle selected from microdisc or nanodisc; andb. exposing the resulting conjugate to a low-frequency AC field having a frequency between approximately 1 Hz to 100 Hz, wherein the low-frequency AC field oscillates the microdisk or nanodisc sufficient to release active TGF-β
from the latent TGF-β
complex. - View Dependent Claims (6)
- from a latent TGF-β
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7. A method of denaturing TGF-β
- , comprising;
a. providing TGF-β
conjugated to a magnetic nanoparticle; andb. exposing the conjugate to a radiofrequency magnetic field effective to denature the TGF-β
that is conjugated to the magnetic nanoparticle. - View Dependent Claims (8, 9)
- , comprising;
Specification