Electron mediator and electrochemical biosensor employing the same

• US 10,094,799 B2
• Filed: 08/24/2015
• Issued: 10/09/2018
• Est. Priority Date: 08/22/2014
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Cho, Seong-je, Kim, Kwang-bok, Kim, Jae-hong, Lee, Su-ho, Jung, Sun-tae, Cho, Jae-geol, Cho, Chul-ho
• Primary Examiner(s): Kaur, Gurpreet
• Application Number:
  US14/834,064
• Publication Number:
  US 20160054254A1
• Time in Patent Office: 1,142 Days
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: H01B 1/02 mainly consisting of metals or alloys

Abstract

Provided is an electron mediator system, which may include a first transition metal complex and a second transition metal complex, wherein a working potential of the electron mediator system may be substantially the same as a molar average of a working potential of the first transition metal complex and a working potential of the second transition metal complex.

Claims

1. An electron mediator system comprising a first transition metal complex and a second transition metal complex, wherein a working potential of the electron mediator system is substantially the same as a molar average of a working potential of the first transition metal complex and a working potential of the second transition metal complex, wherein the first transition metal complex comprises a ligand comprising a thiophene group.

2. The electron mediator system of claim 1, wherein the working potential of the electron mediator system exhibits a linear relationship between the working potential of the first transition metal complex and the working potential of the second transition metal complex according to a molar fraction of the first transition metal complex, wherein a correlation factor (R²) of the linear relationship is 0.95 or greater.

3. The electron mediator system of claim 1, wherein a difference between a primary ionization energy of the first transition metal complex and a primary ionization energy of the second transition metal complex is 15% or less.

4. The electron mediator system of claim 1, wherein the first transition metal complex is 5-(2,5-di-(thiopene-2-yl)-1H-pyrrol-1-yl)-1,10-phenanthroline iron.

5. The electron mediator system of claim 1, wherein the second transition metal complex does not comprise an electrode bonding functional group.

6. The electron mediator system of claim 5, wherein the second transition metal complex is at least one of ferricyanide and hexaamineruthenium (II).

7. The electron mediator system of claim 1, wherein a center metal of the first transition metal complex is the same with a center metal of the second transition metal complex.

8. The electron mediator system of claim 1, wherein the first transition metal complex is 5-(2,5-di-(thiopen-2-yl)-1H-pyrrol-1-yl)-1,10-phenanthroline iron, and the second transition metal complex is ferricyanide.

9. The electron mediator system of claim 1, wherein a center metal of the first transition metal complex is different from a center metal of the second transition metal complex.

10. The electron mediator system of claim 1, wherein the first transition metal complex is 5-(2,5-di-(thiopen-2-yl)-1H-pyrrol-1-yl)-1,10-phenanthroline iron, and the second transition metal complex is hexaamineruthenium (II).

11. A composition for forming a detecting reagent layer comprising:

an electron mediator system comprising a first transition metal complex and a second transition metal complex, wherein a working potential of the electron mediator system is substantially the same as a molar average of a working potential of the first transition metal complex and a working potential of the second transition metal complex;

an enzyme; and

a carrier,

wherein the first transition metal complex comprises a ligand comprising a thiophene group.

12. The composition of claim 11, further comprising a coenzyme.

13. The composition of claim 11, wherein the working potential of the electron mediator system exhibits a linear relationship between the working potential of the first transition metal complex and the working potential of the second transition metal complex according to a molar fraction of the first transition metal complex, wherein a correlation factor (R²) of the linear relationship is 0.95 or greater.

14. The composition of claim 11, wherein a difference between a primary ionization energy of the first transition metal complex and a primary ionization energy of the second transition metal complex is 15% or less.

15. The composition of claim 11, wherein the first transition metal complex is 5-(2,5-di-(thiopene-2-yl)-1H-pyrrol-1-yl)-1,10-phenanthroline iron.

16. A biosensor comprising:

a working electrode formed on an insulating substrate;

a counter electrode formed on the insulating substrate; and

a detecting reagent layer contacting the working electrode and the counter electrode,

wherein the detecting reagent layer comprises an electron mediator system that comprises a first transition metal complex and a second transition metal complex, wherein a working potential of the electron mediator system is substantially the same as a molar average of a working potential of the first transition metal complex and a working potential of the second transition metal complex,

wherein the first transition metal complex comprises a ligand comprising a thiophene group.