Device for generating droplets

• US 10,099,219 B2
• Filed: 11/21/2016
• Issued: 10/16/2018
• Est. Priority Date: 03/25/2010
• Status: Active Grant

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Ness, Kevin D., Kelly, Christopher F., Masquelier, Donald A.
• Primary Examiner(s): WHITE, DENNIS MICHAEL
• Application Number:
  US15/357,840
• Publication Number:
  US 20170065979A1
• Time in Patent Office: 694 Days
• CPC Class Codes: B01F 23/41 Emulsifying
• CPC Class Codes: B01F 33/3011 using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 2035/1034 Transferring microquantities of liquid
• CPC Class Codes: G01N 35/085 Flow Injection Analysis
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y10T 29/494 Fluidic or fluid actuated device making

Abstract

A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

Claims

1. A device for generating droplets, comprising:

a body injection-molded as a single piece and including a bottom surface and a plurality of protrusions located opposite the bottom surface, each protrusion including an annular wall that tapers in thickness in a direction away from the bottom surface, a channel network being formed in the bottom surface and including a first channel, a second channel, and a third channel each arranged parallel to the bottom surface and meeting one another in a droplet-generation region;

a plurality of wells including a carrier well configured to supply carrier fluid to the droplet-generation region via the first channel, a sample well configured to supply sample-containing fluid to the droplet-generation region via the second channel, and a droplet well configured to receive sample-containing droplets in carrier fluid from the third channel, wherein each well of the plurality of wells has an upper portion created by one of the protrusions; and

a sealing member attached to the bottom surface of the body in a fluid-tight seal, and providing a bottom wall for each channel.

2. The device of claim 1, wherein the plurality of wells includes a row of carrier wells, a row of sample wells, and a row of droplet wells, and wherein the sealing member provides a floor for each well of the plurality of wells.

3. The device of claim 2, wherein each row has exactly eight wells.

4. The device of claim 2, wherein each carrier well is disposed on a same line as one of the sample wells and one of the droplet wells.

5. The device of claim 2, wherein the row of sample wells is located intermediate the row of carrier wells and the row of droplet wells.

6. The device of claim 1, wherein the sealing member is a substantially featureless film.

7. The device of claim 1, wherein the device comprises another well that is fluidically connected to the droplet well via the channel network.

8. The device of claim 1, wherein the upper portion of at least one well of the plurality of wells forms a rim having a non-circular outer perimeter.

9. A method of generating droplets with the device of claim 1, the method comprising:

placing a discrete volume of carrier fluid into the carrier well, and a discrete volume of sample-containing fluid into the sample well; and

creating a pressure differential after the step of placing to drive generation of sample-containing droplets in the droplet-generation region and travel of the sample-containing droplets to the droplet well.

10. A device for generating droplets, comprising:

a substantially planar substrate having a top surface opposite a bottom surface;

a plurality of protrusions injection-molded along with the substrate and projecting upwardly from the top surface of the substrate;

an array of wells including a row of carrier wells configured to supply carrier fluid, a row of sample wells configured to supply sample-containing fluid, and a row of droplet wells configured to collect sample-containing droplets disposed in carrier fluid, wherein each well of the array of wells has an upper portion created by one of the protrusions, and wherein the carrier wells are larger than the sample wells and smaller than the droplet wells;

a plurality of separate channel networks formed in the bottom surface of the substrate, each channel network interconnecting one of the carrier wells, one of the sample wells, and one of the droplet wells, and including a first channel, a second channel, and a third channel that meet one another in a droplet-generation region; and

a sealing member attached to the bottom surface of the substrate in a fluid-tight seal, the sealing member providing a floor for each well and a bottom wall for each channel.

11. The device of claim 10, wherein each row has exactly eight wells.

12. The device of claim 10, wherein each well includes an annular wall that tapers in thickness in a direction away from the substrate.

13. The device of claim 10, wherein each sample well has an inside diameter that increases smoothly in a direction away from the substrate.

14. The device of claim 10, wherein each sample well has an outside diameter that decreases in a direction away from the substrate.

15. The device of claim 10, wherein the row of sample wells is located intermediate the row of carrier wells and the row of droplet wells.

16. The device of claim 10, wherein the sealing member is a substantially featureless film.

17. The device of claim 10, wherein the sealing member is thinner than the substrate.

18. The device of claim 10, wherein the one carrier well, the one sample well, and the one droplet well interconnected by the channel network are arranged on a same line.

19. The device of claim 18, wherein the line is orthogonal to each of the rows.

20. The device of claim 10, wherein a pair of channels of the channel network connect the one carrier well to the droplet-generation region and extend separately to the droplet-generation region.

21. The device of claim 10, wherein each protrusion has a wall thickness of 0.04 to 0.4 inches.

22. The device of claim 10, wherein each droplet well has a rim formed at a top of the droplet well by one of the protrusions.

23. The device of claim 22, wherein the rim has a non-circular outer perimeter.

24. A method of generating droplets with the device of claim 10, the method comprising:

placing a discrete volume of carrier fluid into each carrier well, and a discrete volume of sample-containing fluid into each sample well; and

creating a pressure differential after the step of placing, to drive generation of sample-containing droplets in each droplet-generation region and collection of sample-containing droplets in each droplet well.

25. A device for generating droplets, comprising:

a body injection-molded as a single piece and including a substrate having a bottom surface, the body also including a plurality of annular protrusions projecting from the substrate opposite the bottom surface, a channel network being formed in the bottom surface and including a first channel, a second channel, and a third channel each arranged parallel to the bottom surface;

a plurality of wells including a carrier well configured to supply carrier fluid, a sample well configured to supply sample-containing fluid, and a droplet well configured to collect sample-containing droplets disposed in carrier fluid, wherein each well has an upper portion created by one of the protrusions; and

a sealing member attached to the bottom surface of the substrate in a fluid-tight seal, the sealing member providing a bottom wall for each channel;

wherein the first channel, the second channel, and the third channel meet one another in a droplet-generation region, and wherein the carrier well is fluidically connected to the droplet-generation region via the first channel, the sample well is fluidically connected to the droplet-generation region via the second channel, and the droplet well is fluidically connected to the droplet-generation region via the third channel.