Method for inducing differentiation of pluripotent stem cells into endodermal cells
First Claim
1. A composition for inducing differentiation of pluripotent stem cells into hepatocytes and/or liver precursor cells, comprising:
- a pharmaceutically acceptable carrier; and
a synthetic peptide comprising;
a stem cell differentiation-inducing peptide sequence that induces differentiation of pluripotent stem cells into hepatocytes and/or liver precursor cells, anda transmembrane peptide sequence at the N end of or C end of the amino acid sequence of the stem cell differentiation-inducing peptide sequence,wherein the total number of amino acid residues constituting the synthetic peptide is 100 or less amino acid residues,the transmembrane peptide sequence is selected from the amino acid sequences represented by any of SEQ ID NOs;
7 to 15, andthe stem cell differentiation-inducing peptide sequence is selected from the amino acid sequences of i) through iv) below;
i) Amino acid sequence of SEQ ID NO;
1 below;
1 Assignment
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Accused Products
Abstract
A synthetic peptide having a stem cell differentiation-inducing activity to induce differentiation of pluripotent stem cells into endodermal cells, a stem cell differentiation inducer having this peptide as an active ingredient, and a method for inducing differentiation of pluripotent stem cells using these. The synthetic peptide provided by the present invention contains a stem cell differentiation-inducing peptide sequence, and this stem cell differentiation-inducing peptide sequence is selected from (1) an amino acid sequence constituting a signal peptide in any of amyloid precursor proteins (APP), amyloid precursor-like protein (APLP) 1 and APLP2, which are known as proteins belonging to the APP family, (2) a partial amino acid sequence constituting the signal peptide, or (3) a modified amino acid sequence formed by substitution, deletion and/or addition of 1, 2 or 3 amino acid residues in these amino acid sequences.
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Citations
8 Claims
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1. A composition for inducing differentiation of pluripotent stem cells into hepatocytes and/or liver precursor cells, comprising:
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a pharmaceutically acceptable carrier; and a synthetic peptide comprising; a stem cell differentiation-inducing peptide sequence that induces differentiation of pluripotent stem cells into hepatocytes and/or liver precursor cells, and a transmembrane peptide sequence at the N end of or C end of the amino acid sequence of the stem cell differentiation-inducing peptide sequence, wherein the total number of amino acid residues constituting the synthetic peptide is 100 or less amino acid residues, the transmembrane peptide sequence is selected from the amino acid sequences represented by any of SEQ ID NOs;
7 to 15, andthe stem cell differentiation-inducing peptide sequence is selected from the amino acid sequences of i) through iv) below; i) Amino acid sequence of SEQ ID NO;
1 below; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification