Compositions and methods for controlling arthropod parasite and pest infestations

• US 10,100,306 B2
• Filed: 12/14/2016
• Issued: 10/16/2018
• Est. Priority Date: 11/04/2013
• Status: Active Grant

• Current Assignee: GreenLight Biosciences, Inc., United States Department Of Agriculture
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Inberg, Alex, Kapoor, Mahak, Evans, Jay
• Primary Examiner(s): VIVLEMORE, TRACY ANN
• Application Number:
  US15/378,513
• Publication Number:
  US 20170088838A1
• Time in Patent Office: 671 Days
• Field of Search: None
• CPC Class Codes: A01K 51/00 Appliances for treating beehives or parts thereof, e.g. for cleaning or disinfecting
• CPC Class Codes: A01N 37/46 N-acyl derivatives
• CPC Class Codes: A01N 63/14 Insects
• CPC Class Codes: A01N 63/16 Arachnids
• CPC Class Codes: A01N 63/60 Isolated nucleic acids
• CPC Class Codes: A61K 31/713 Double-stranded nucleic acids or oligonucleotides
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/14 interfering N.A.
• CPC Class Codes: C12N 2310/531 Stem-loop; Hairpin

Abstract

This application provides and discloses anti-parasitic, anti-pest or insecticidal nucleic acid molecules and their calmodulin target genes for the control of arthropod parasites and pests. This application further provides methods and compositions for the control and treatment of parasites and pests in Apis mellifera (honey bee) hives.

Claims

1. A method of reducing the parasitation of a honeybee by Varroa destructor, comprising providing the bee an effective amount a nucleic acid composition, wherein said nucleic acid is essentially identical or essentially complementary to a region of a Varroa destructor calmodulin gene sequence, or an RNA transcribed therefrom, thereby reducing the parasitation of said bee by Varroa destructor.

2. The method of claim 1, wherein said honeybee is a forager or a hive bee.

3. The method of claim 1, wherein said honeybee is a bee of a colony and said feeding reduces the parasitation of said bee colony by Varroa destructor.

17. The method of claim 3, wherein the method treats or prevents Colony Collapse Disorder in said colony.

18. The method of claim 17, wherein said nucleic acid molecule is a dsRNA.

19. The method of claim 17, wherein said calmodulin gene sequence has at least 80% sequence identity to a sequence selected from SEQ ID NOs:

1 and 3, or wherein said calmodulin gene sequence comprises at least 18 contiguous nucleotides of a sequence selected from SEQ ID NOs;

1 and 3.

20. The method of claim 18, wherein said dsRNA sequence is SEQ ID NO:

3.

4. A method of reducing the parasite load of a honeybee hive, comprising providing said hive an effective amount of a nucleic acid that is essentially identical or essentially complementary to a region of a parasite calmodulin gene sequence, or an RNA transcribed therefrom, whereby the parasite load of said hive is reduced.

5. The method of claim 4, wherein said parasite is Varroa destructor.

6. The method of claim 4, wherein said honeybee hive has an initial parasite load of at least 1, 2, 3, 5, 10, or more parasites per 100 bees.

7. A method of selectively treating an arthropod species for parasites, comprising delivering an effective amount of a nucleic acid that is essentially identical or essentially complementary to a region of a parasite calmodulin gene sequence, or an RNA transcribed therefrom, to an arthropod species.

8. The method of claim 7, wherein said treatment decreases the parasitic load of said arthropod species, reduces the death of said arthropod species, or prevents parasitation of said arthropod species.

9. The method of claim 7, wherein said arthropod species is selected from the group consisting of Apis mellifera, Apis cerana, Trigona minima, Halictidae, Bombus sp., Ichneumonoidea (parasitic wasps), fleas, flies, lice, ticks, and mites.

10. The method of claim 7, wherein said arthropod species is a colony species.

11. The method of claim 10, wherein said arthropod species is Apis mellifera.

12. The method of claim 7, wherein said parasites are selected from the group consisting of Acari (ticks, mites), Hippoboscoidea (flies), Ichneumonoidea (parasitic wasps), Oestridae (bot flies), Phthiraptera (lice), Siphonaptera (fleas), Tantulocarida, Pea crab, and Sacculina.

13. The method of claim 12, wherein said parasite is a mite or a tick.

14. The method of claim 13, wherein said mite or a tick is a Tropilaelap mite, a deer tick, or a two-spotted spider mite.

15. The method of claim 13, wherein said parasite is Varroa destructor.

16. The method of claim 7, wherein said delivering comprises delivery through a feeder, spraying on the hive frames, or delivery by contact using an intra-hive device impregnated with said composition.