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Aptamers comprising CPG motifs

  • US 10,100,316 B2
  • Filed: 04/21/2004
  • Issued: 10/16/2018
  • Est. Priority Date: 11/21/2002
  • Status: Active Grant
First Claim
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1. An aptamer comprising a first nucleic acid sequence that binds to a first target and a second nucleic acid sequence that binds to a second target, wherein the first sequence does not stimulate an immune response upon binding to the first target;

  • and the second sequence is an immunostimulatory CpG motif that stimulates an immune response, wherein the CpG motif consists of an oligonucleotide sequence selected from the group consisting of (T/U)GAACG(T/U)(T/U)CGAGA(T/U) (SEQ ID NO;

         13), AACG(T/U)(T/U)CGAGA(T/U) (SEQ ID NO;

         14), AACG(T/U)(T/U)CGAG (SEQ ID NO;

         15) and G(T/U)GAACG(T/U)(T/U)CGAG (SEQ ID NO;

         16), wherein the first sequence binds to a first target, wherein the first target is Tumor Necrosis Factor (TNF-α

    , and wherein the aptamer comprises the structural arrangement in a 5′

    to 3′

    direction;

    second nucleic acid sequence-first nucleic acid sequence-3′

    InvdT, where 3′

    InvdT represents a 3′

    inverted deoxythymidine.

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