Tailored oils produced from recombinant oleaginous microorganisms
First Claim
1. A method for producing an oil comprising triacylglycerides, or a product produced from the oil, the method comprising:
- cultivating a cell of a recombinant microalga of the genera Prototheca, the cell comprising recombinant nucleic acids operable to decrease or eliminate the expression of a gene encoding a β
-ketoacyl-ACP synthase I enzyme via homologous recombination, wherein the homologous recombination interrupts or replaces a gene encoding the β
-ketoacyl-ACP synthase I enzyme; and
recovering the oil from the cell, and optionally further processing the oil to produce a food, fuel, or chemical product,wherein the oil has an altered fatty acid profile due to the recombinant nucleic acids.
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Abstract
Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
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Citations
6 Claims
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1. A method for producing an oil comprising triacylglycerides, or a product produced from the oil, the method comprising:
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cultivating a cell of a recombinant microalga of the genera Prototheca, the cell comprising recombinant nucleic acids operable to decrease or eliminate the expression of a gene encoding a β
-ketoacyl-ACP synthase I enzyme via homologous recombination, wherein the homologous recombination interrupts or replaces a gene encoding the β
-ketoacyl-ACP synthase I enzyme; andrecovering the oil from the cell, and optionally further processing the oil to produce a food, fuel, or chemical product, wherein the oil has an altered fatty acid profile due to the recombinant nucleic acids. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification