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Tailored oils produced from recombinant oleaginous microorganisms

  • US 10,100,341 B2
  • Filed: 12/18/2015
  • Issued: 10/16/2018
  • Est. Priority Date: 02/02/2011
  • Status: Active Grant
First Claim
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1. A method for producing an oil comprising triacylglycerides, or a product produced from the oil, the method comprising:

  • cultivating a cell of a recombinant microalga of the genera Prototheca, the cell comprising recombinant nucleic acids operable to decrease or eliminate the expression of a gene encoding a β

    -ketoacyl-ACP synthase I enzyme via homologous recombination, wherein the homologous recombination interrupts or replaces a gene encoding the β

    -ketoacyl-ACP synthase I enzyme; and

    recovering the oil from the cell, and optionally further processing the oil to produce a food, fuel, or chemical product,wherein the oil has an altered fatty acid profile due to the recombinant nucleic acids.

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