Optical emission collection and detection device and method

• US 10,101,273 B2
• Filed: 08/07/2015
• Issued: 10/16/2018
• Est. Priority Date: 02/17/2012
• Status: Active Grant

• Current Assignee: Teledyne FLIR Detection Incorporated (Teledyne Technologies Incorporated)
• Original Assignee: Flir Detection, Inc. (Teledyne Technologies Incorporated)
• Inventors: Ja, Shiou-jyh
• Primary Examiner(s): Decenzo, Shawn
• Application Number:
  US14/821,553
• Publication Number:
  US 20160033410A1
• Time in Patent Office: 1,166 Days
• Field of Search: None
• CPC Class Codes: G01N 2021/6423 Spectral mapping, video display
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 33/227 Explosives, e.g. combustive properties thereof detecting explosives in air G01N33/0057

Abstract

This invention generally relates to optical devices that can collect and detect signal emissions effectively while allowing the excitation light path and the sample flow path to coexist non-obstructively in a compact format. The device has various embodiments, such as an embodiment including a plurality of reporters disposed on a sensing surface, wherein each one of the plurality of reporters is configured to react with a least one target analyte, a hyperspectral detection module configured to capture hyperspectral image data corresponding to the plurality of reporters, and a controller. The controller is configured to receive the hyperspectral image data from the hyperspectral detection module and generate a temporal spectral signature corresponding to each one of the plurality of reporters from the received hyperspectral image data.

Claims

1. A device comprising:

a plurality of reporters disposed on a sensing surface, wherein each one of the plurality of reporters is configured to react with a least one target analyte;

a hyperspectral detection module configured to capture hyperspectral image data corresponding to the plurality of reporters, wherein the hyperspectral detection module comprises a dielectric wedge interferometer structure configured to produce a plurality of interferograms corresponding to the plurality of reporters without scanning the dielectric wedge interferometer structure across the plurality of reporters; and

a controller configured to;

receive the hyperspectral image data from the hyperspectral detection module; and

generate a temporal spectral signature corresponding to each one of the plurality of reporters from the received hyperspectral image data.

2. The device of claim 1, wherein:

the plurality of reporters comprises at least two different reporter materials; and

the plurality of reporters comprises an array of reporter strips or an array of reporter patches.

3. The device of claim 1, further comprising:

an excitation assembly configured to provide optical excitation of at least one of the plurality of reporters via a light source configured to emit an excitation wavelength, wherein the at least one of the plurality of reporters comprises a fluorescing reporter.

4. The device of claim 1, further comprising:

an input segment configured to receive an analyte transport fluid, wherein the sensing surface is in fluid communication with the input segment.

5. The device of claim 1, wherein:

the hyperspectral image data comprises the plurality of interferograms; and

the controller is configured to generate the temporal spectral signature by processing each one of a time series of the plurality of interferograms, corresponding to one of the plurality of reporters, to extract a time series of spectrums of the one of the plurality of reporters.

6. The device of claim 1, wherein:

the dielectric wedge interferometer structure comprises two glass plates that are at least partially separated by a linearly varying gap; and

the air-wedge interferometer structure is integrated with a detector of the hyperspectral detector module.

7. The device of claim 5, wherein:

the plurality of reporters comprise an array of reporter strips;

a length of a gap of the dielectric wedge interferometer structure is aligned with lengths of the array of reporter strips; and

the controller is configured to apply a fast Fourier transform to the hyperspectral image data to generate each temporal spectral signature.

8. The device of claim 1, wherein:

the hyperspectral detector module comprises a lens assembly implemented as a multi-element lens arrangement configured to receive light comprising emission angles up to approximately 35-45 degrees, half angle, emitted by the plurality of reporters and to focus the received light onto a detector of the hyperspectral detector module.

9. A method comprising:

receiving hyperspectral image data from a hyperspectral detection module, wherein the hyperspectral detection module is configured to capture hyperspectral image data corresponding to a plurality of reporters disposed on a sensing surface, the hyperspectral detection module comprises a dielectric wedge interferometer structure configured to produce a plurality of interferograms corresponding to the plurality of reporters without scanning the dielectric wedge interferometer structure across the plurality of reporters, and each one of the plurality of reporters is configured to react with a least one target analyte; and

generating a temporal spectral signature corresponding to each one of the plurality of reporters from the received hyperspectral image data.

10. The method of claim 9, wherein:

the plurality of reporters comprises at least two different reporter materials;

the plurality of reporters comprises an array of reporter strips or an array of reporter patches; and

the method further comprises determining a presence and/or type of the at least one target analyte from the temporal spectral signatures.

11. The method of claim 9, further comprising:

providing optical excitation of at least one of the plurality of reporters via a light source configured to emit an excitation wavelength, wherein the at least one of the plurality of reporters comprises a fluorescing reporter.

12. The method of claim 9, wherein:

the hyperspectral image data comprises the plurality of interferograms; and

the generating the temporal spectral signature comprises processing each one of a time series of the plurality of interferograms, corresponding to one of the plurality of reporters, to extract a time series of spectrums of the one of the plurality of reporters.

13. The method of claim 9, wherein:

the dielectric wedge interferometer structure comprises two glass plates that are at least partially separated by a linearly varying gap; and

the air-wedge interferometer structure is integrated with a detector of the hyperspectral detector module.

14. The method of claim 12, wherein:

the plurality of reporters comprise an array of reporter strips;

a length of a gap of the dielectric wedge interferometer structure is aligned with lengths of the array of reporter strips; and

the generating the temporal spectral signature comprises applying a fast Fourier transform to the hyperspectral image data.

15. The method of claim 9, wherein:

the hyperspectral detector module comprises a lens assembly implemented as a multi-element lens arrangement configured to receive light comprising emission angles up to approximately 35-45 degrees, half angle, emitted by the plurality of reporters and to focus the received light onto a detector of the hyperspectral detector module.