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Optimal soybean loci

  • US 10,106,804 B2
  • Filed: 01/11/2018
  • Issued: 10/23/2018
  • Est. Priority Date: 11/04/2013
  • Status: Active Grant
First Claim
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1. A recombinant soybean cell produced by the following steps:

  • a. providing a soybean cell wherein said cell comprises a target nongenic soybean genomic nucleic acid molecule selected from the group consisting ofSEQ ID NO;

    639, identified as soy_OGL_1423,SEQ ID NO;

    137, identified as soy_OGL_1434,SEQ ID NO;

    76, identified as soy_OGL_4625,SEQ ID NO;

    440, identified as soy_OGL_6362,SEQ ID NO;

    43, identified as soy_OGL_308,SEQ ID NO;

    566, identified as soy_OGL_307,SEQ ID NO;

    4236, identified as soy_OGL_310,SEQ ID NO;

    47, identified as soy_OGL_684,SEQ ID NO;

    2101, identified as soy_OGL_682, andSEQ ID NO;

    48, identified as soy_OGL_685b. selecting a site specific nuclease that specifically binds and cleaves said target nongenic soybean genomic nucleic acid molecule;

    c. introducing said site specific nuclease activity into said soybean cell;

    d. introducing a DNA of interest into the soybean cell;

    e. inserting the DNA of interest into said target nongenic soybean genomic nucleic acid molecule; and

    ,f. selecting transgenic soybean cells comprising the DNA of interest targeted to said nongenic nucleic acid molecule.

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