Optical coherence photoacoustic microscopy

• US 10,107,613 B2
• Filed: 08/30/2016
• Issued: 10/23/2018
• Est. Priority Date: 06/15/2011
• Status: Active Grant

• Current Assignee: Northwestern University, University of Southern California
• Original Assignee: Northwestern University, University of Southern California
• Inventors: Jiao, Shuliang, Zhang, Hao
• Primary Examiner(s): Bologna, Dominic J
• Application Number:
  US15/251,610
• Publication Number:
  US 20160370167A1
• Time in Patent Office: 784 Days
• CPC Class Codes: G01B 9/0203 With imaging systems
• CPC Class Codes: G01B 9/02091 Tomographic interferometers, e.g. based on optical coherence
• CPC Class Codes: G01N 29/0681 by acoustic microscopy, e.g. scanning acoustic microscopy
• CPC Class Codes: G01N 29/2418 using optoacoustic interaction with the material, e.g. laser radiation, photoacoustics photoacoustic cells G01N21/1702; measuring characteristics of vibrations by using radiation-sensitive means G01H9/00; acousto-optical conversion techniques for short-range imaging G01S15/8965; sound-producing devices using laser bundle G10K15/046

Abstract

A system and method for providing an optical coherence photoacoustic (OC-PAM) microscopy. An OC-PAM microscope includes a light source that outputs light, a scanner, a detector, a transducer, and an image processing module. The scanner receives the light and scans the light across a sample. The detector receives reflected light from the sample in response to the scanned light. The transducer detects photoacoustic waves induced in the sample by the scanned light. The image processing module receives output from the detector and the transducer and generates a photoacoustic microscopy (PAM) image and an optical coherence tomography (OCT) image based on the received output from the detector and the transducer. The PAM and OCT image data may be fused to form a single, OC-PAM image. Additionally, a series of PAM images and OCT images, respectively, may be combined to generate three-dimensional PAM and OCT images, respectively.

Claims

1. A method of optical coherence photoacoustic multi-modal microscopic imaging of a target, the method comprising:

generating, substantially simultaneously using a light source, A-scans for optical coherence tomography and photoacoustic microscopy of the target;

forming, using a processor, B-scan images for optical coherence tomography and photoacoustic microscopy of the target co-registered in a lateral direction and both formed from photons generated from the A-scans; and

generating, using the processor, a fused image of the target from B-scan image data for optical coherence tomography and photoacoustic microscopy, wherein the photoacoustic microscopy B-scan image data is scaled and interpolated to be co-registered with the B-scan image for optical coherence tomography in a depth direction and to fuse with the optical coherence tomography B-scan data to form a combined optical coherence tomography and photoacoustic microscopy image.

2. The method of claim 1, wherein the light source is one of a pulsed broadband laser that outputs pulsed broadband light and a swept laser source that outputs pulsed swept laser light of a plurality of wavelengths, wherein the wavelengths are swept in a scan period shorter than 10 nanoseconds.

3. The method of claim 2, wherein the light source is the swept laser source and the detected photoacoustic waves have energy from the sum of the pulsed swept laser light of the plurality of wavelengths in the scan period.

4. The method of claim 1, further including moving the light source to a second point on the target to obtain additional A-scans for optical coherence tomography and photoacoustic microscopy of the target.

5. The method of claim 4, wherein moving the light source to a second point on the target includes scanning the light via an x-y scanner to direct light with respect to the target.

6. The method of claim 1, further including splitting, via a beam splitter, the light into a sample arm light and a reference arm light.

7. The method of claim 6, further including:

partially reflecting and partially transmitting the reference arm light;

detecting, with a photodiode, the reflected reference arm light; and

outputting, based on detecting the reflected light with the photodiode, a trigger signal to initiate data acquisition for the photoacoustic microscopy A-scan.

8. The method of claim 7, further including combining the reflected light, before reaching the detector, with the partially transmitted reference arm light for the optical coherence tomography A-scan.

9. An optical coherence photoacoustic microscopy controller apparatus comprising:

a memory storing instructions for execution; and

a computing device configured to execute the instructions stored in the memory to least;

generate, substantially simultaneously using a light source, A-scans for optical coherence tomography and photoacoustic microscopy of a target;

form B-scan images for optical coherence tomography and photoacoustic microscopy of the target co-registered in a lateral direction and both formed from photons generated from the A scans; and

generate a fused image of the target from B-scan image data for optical coherence tomography and photoacoustic microscopy, wherein the photoacoustic microscopy B-scan image data is scaled and interpolated to be co-registered with the B-scan image for optical coherence tomography in a depth direction and to fuse with the optical coherence tomography B-scan data to form a combined optical coherence tomography and photoacoustic microscopy image.

10. The apparatus of claim 9, wherein the light source is one of a pulsed broadband laser that outputs pulsed broadband light and a swept laser source that outputs pulsed swept laser light of a plurality of wavelengths, wherein the wavelengths are swept in a scan period shorter than 10 nanoseconds.

11. The apparatus of claim 10, wherein the light source is the swept laser source and the detected photoacoustic waves have energy from the sum of the pulsed swept laser light of the plurality of wavelengths in the scan period.

12. The apparatus of claim 9, wherein the instructions, when executed, further configure the computing device to move the light source to a second point on the target to obtain additional A-scans for optical coherence tomography and photoacoustic microscopy of the target.

13. The apparatus of claim 12, wherein moving the light source to a second point on the target includes scanning the light via an x-y scanner to direct light with respect to the target.

14. The apparatus of claim 9, wherein the instructions, when executed, further configure the computing device to split, via a beam splitter, the light into a sample arm light and a reference arm light.

15. The apparatus of claim 14, wherein the instructions, when executed, further configure the computing device to:

partially reflect and partially transmit the reference arm light;

detect, with a photodiode, the reflected reference arm light; and

output based on detecting the reflected light with the photodiode, a trigger signal to initiate data acquisition for the photoacoustic microscopy A-scan.

16. The apparatus of claim 15, wherein the instructions, when executed, further configure the computing device to combine the reflected light, before reaching the detector, with the partially transmitted reference arm light for the optical coherence tomography A-scan.