Collection of suspended cells using a transferable membrane
First Claim
1. A device for collecting cells of interest from a sample comprising:
- a sample collection layer comprising a porous membrane, wherein the porous membrane is configured to receive the sample and collect the cells of interest;
a support layer comprising (1) an application zone in fluidic communication with the sample collection layer, (2) a channel extending along the support layer, wherein the channel is in fluidic communication with the application zone, and (3) a first outlet at a distal end of the channel, the first outlet defining a first flow path through which the sample flows out of the device; and
a solid support in fluidic communication with the support layer, wherein the solid support comprises a second outlet defining a second flow path through which the sample flows out of the device,wherein the sample collection layer or the support layer is removably coupled to the solid support.
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Accused Products
Abstract
Devices, methods, and kits directed towards collecting and preparing cells using a separable sample collection layer may be configured to collect or treat cells from a liquid sample with mechanisms for easy transfer of the cells prior to analysis or imaging. The separable sample collection layer may comprise a porous membrane that cells may be collected on, and one or more support layers comprising tape with one or more adhesive coatings and release liner. The devices, methods and kits may be configured with support layers comprising cutouts that form vertically or horizontally oriented microchannels for efficiently removing undesirable liquid. Following collection and/or treatment, cells collected onto the porous membrane may be adhered to another surface for further processing or analysis.
418 Citations
27 Claims
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1. A device for collecting cells of interest from a sample comprising:
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a sample collection layer comprising a porous membrane, wherein the porous membrane is configured to receive the sample and collect the cells of interest; a support layer comprising (1) an application zone in fluidic communication with the sample collection layer, (2) a channel extending along the support layer, wherein the channel is in fluidic communication with the application zone, and (3) a first outlet at a distal end of the channel, the first outlet defining a first flow path through which the sample flows out of the device; and a solid support in fluidic communication with the support layer, wherein the solid support comprises a second outlet defining a second flow path through which the sample flows out of the device, wherein the sample collection layer or the support layer is removably coupled to the solid support. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification