Collector architecture layout design

• US 10,112,198 B2
• Filed: 08/26/2015
• Issued: 10/30/2018
• Est. Priority Date: 08/26/2014
• Status: Active Grant

• Current Assignee: Academia Sinica (Government of The Republic of China)
• Original Assignee: Academia Sinica (Government of The Republic of China)
• Inventors: Chang, Ying-Chih, Lai, Jr-Ming, Wu, Jen-Chia
• Primary Examiner(s): Wecker, Jennifer
• Application Number:
  US14/836,390
• Publication Number:
  US 20160059234A1
• Time in Patent Office: 1,161 Days
• CPC Class Codes: B01L 2200/0652 Sorting or classification of particles or molecules
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/08 Geometry, shape and general structure
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28

Abstract

The disclosure provides for compositions and methods for the collection of rare cells using an interspersed microstructure design.

Claims

1. A microfluidic channel comprising:

a plurality of microstructures within the channel;

wherein the plurality of microstructures is arranged in a plurality of columns substantially parallel to one another, wherein the plurality of columns comprise at least four columns comprising a first column adjacent to a second column, the second column adjacent to a third column, and the third column adjacent to a fourth column;

wherein the number of microstructures in the first column is greater than the number of microstructures in the second column or the third column; and

wherein the number of microstructures in the fourth column is greater than the number of microstructures in the second column or the third column; and

a plurality of vortex regions at which one or more vortexes are generated in response to fluid flow, wherein each vortex region of the plurality is substantially free of the plurality of microstructures and comprises at least a cylindrical volume having (1) a height of the channel and (2) a base having a diameter at least 20% a width of the channel, wherein the plurality of vortex regions of the plurality are separated from each other by at least one microstructure along a length of the channel; and

wherein said vortex regions are configured to increase the mixing of particles of interest and thereby to increase the likelihood of binding particles of interest to a microstructure.

2. The channel of claim 1, wherein each vortex region of the plurality comprises at least a rectangular volume having (1) a height of the channel, (2) a width equal to the diameter, and (3) a length at least 30% a width of the channel.

3. The channel of claim 1, wherein the plurality of vortex regions are positioned in a palindromic pattern along the length of the channel.

4. The channel of claim 1, wherein the plurality of vortex regions are positioned in a repeating pattern along the length of the channel.

5. The channel of claim 1, wherein the plurality of microstructures are arranged in a plurality of columns substantially parallel to one another and wherein each column of the plurality of columns comprises a column length equal to a distance from an outermost edge of a first microstructure to an outermost edge of a last microstructure in the column.

6. The channel of claim 5, wherein the plurality of columns comprise columns having a first length and columns having a second length greater than the first length, and wherein the first length is equal to or less than 60% of the second length.

7. The channel of claim 5, wherein the plurality of columns comprise columns having a first length and columns having a second length greater than the first length, and wherein each column having the first length is adjacent to at least another column having the first length.

8. The channel of claim 1, wherein the channel comprises a minimum distance between ends of microstructures measured along an axis parallel to a channel width and a maximum distance between ends of microstructures measured along the axis parallel to the channel width, and wherein the minimum distance is equal to or less than 60% of the maximum distance.

9. The channel of claim 5, wherein the each column of the plurality comprises a linear arrangement of microstructures perpendicular to the fluid flow pathway.

10. The channel of claim 7, wherein the plurality of columns are arranged in pattern of columns having 3, 2, 1, 1, 2, 3, 2, 1, 1, 2, 3 microstructures.

11. The channel of claim 1, wherein the channel is coated with a non-fouling layer and a set of binding moieties configured to selectively bind particles of interest.

12. The channel of claim 1, wherein the one or more vortexes are two dimensional vortexes.

13. The channel of claim 1, wherein the one or more vortexes are three dimensional vortexes.

14. The channel of claim 5, wherein a center of each column of the plurality of columns aligns with one another within the channel.

15. The channel of claim 1, wherein the one or more vortexes are generated at regular intervals along the length of the channel.

16. The channel of claim 5, wherein the column length is measured along a width of the channel, and wherein the plurality of columns comprise columns having a minimum length and columns having a maximum length greater than the minimum length.

17. The channel of claim 16, wherein each column having the minimum length comprises a single microstructure.

18. The channel of claim 16, wherein each column having the maximum length comprises three microstructures.

19. The channel of claim 1, wherein each of the plurality of vortex regions is separated from another by at least one whole microstructure along the length of the channel.

20. The microfluidic device of claim 1, wherein the number of microstructures in the second column is the same as the number of microstructures in the third column.

21. The microfluidic device of claim 1, wherein the number of microstructures in the first column is greater than the number of microstructures in the second column and the number of microstructures in the first column is greater than the number of microstructures in the third column.

22. The microfluidic device of claim 1, wherein the number of microstructures in the fourth column is greater than the number of microstructures in the second column and the number of microstructures in the fourth column is greater than the number of microstructures the third column.

23. The microfluidic device of claim 21, wherein the number of microstructures in the fourth column is greater than the number of microstructures in the second column and the number of microstructures in the fourth column is greater than the number of microstructures the third column.

24. The microfluidic device of claim 1, wherein the number of microstructures in the first column is greater than the number of microstructures in the third column and the number of microstructures in the first column is equal to the number of microstructures in the second column.