Compositions and methods for sequencing nucleic acids

• US 10,112,969 B2
• Filed: 05/08/2017
• Issued: 10/30/2018
• Est. Priority Date: 06/02/2010
• Status: Active Grant

• Current Assignee: Industrial Technology Research Institute
• Original Assignee: Industrial Technology Research Institute
• Inventors: Chiou, Chung-Fan, Pan, Chao-Chi, Fann, Jenn-Yeh, Chang, Shang-Chia, Fu, Tsu-Ju
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US15/589,176
• Publication Number:
  US 20180111959A1
• Time in Patent Office: 540 Days
• Field of Search: 536 231, 536 266, 435 61, 435 911
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2525/117 incorporating modified base
• CPC Class Codes: C12Q 2525/119 incorporating abasic sites
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels

Abstract

Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing.

Claims

1. A compound having Formula I:

2. The compound of claim 1, wherein R₃ is chosen from:

3. The compound of claim 1, wherein R₃ is chosen from:

4. The compound of claim 2, wherein n is 1, R₁ is

5. The compound of claim 2, wherein Y₁ is S⁻

.

6. The compound of claim 2, wherein R₃ is

7. The compound of claim 2, wherein Y₃ is O⁻

.