Tissue ablation with irreversible electroporation

• US 10,117,701 B2
• Filed: 03/05/2015
• Issued: 11/06/2018
• Est. Priority Date: 12/24/2003
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Davalos, Rafael, Rubinsky, Boris
• Primary Examiner(s): Hupczey, Jr., Ronald
• Application Number:
  US14/639,632
• Publication Number:
  US 20150173824A1
• Time in Patent Office: 1,342 Days
• CPC Class Codes: A61B 18/12 by passing a current through the tissue to be heated, e.g. high-frequency current
• CPC Class Codes: A61B 18/1233 with circuits for assuring patient safety
• CPC Class Codes: A61B 18/1477 Needle-like probes
• CPC Class Codes: A61B 2018/00577 Ablation
• CPC Class Codes: A61B 2018/00613 Irreversible electroporation reversible electroporation A61N1/327
• CPC Class Codes: A61B 2018/00714 Temperature
• CPC Class Codes: A61B 2018/00761 Duration
• CPC Class Codes: A61B 2018/00827 Current
• CPC Class Codes: A61B 2018/1425 Needle
• CPC Class Codes: A61N 1/0412 Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
• CPC Class Codes: A61N 1/0472 Structure-related aspects
• CPC Class Codes: A61N 1/05 for implantation or insertion into the body, e.g. heart electrode A61N1/06 takes precedence
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation

Abstract

A new method for the ablation of undesirable tissue such as cells of a cancerous or non-cancerous tumor is disclosed. It involves the placement of electrodes into or near the vicinity of the undesirable tissue through the application of electrical pulses causing irreversible electroporation of the cells throughout the entire area of the undesirable tissue. The electric pulses irreversibly permeate the cell membranes, thereby invoking cell death. The irreversibly permeabilized cells are left in situ and are removed by the body immune system. The amount of tissue ablation achievable through the use of irreversible electroporation without inducing thermal damage is considerable.

Claims

1. A device for ablating tissue, comprising:

first and second electrodes configured to-position a tissue zone to be treated there between;

a voltage generator in electrical connection with the first and second electrodes, and configured for applying a voltage between the first and second electrodes in a manner which provides a predetermined electric field around the tissue zone for a time sufficient to perform irreversible electroporation to kill substantially all of the cells in the identified tissue zone; and

a voltage regulator in electrical connection with the voltage generator and configured for adjusting the voltage and pulse duration of the voltage generator to obtain irreversible electroporation of cells in the tissue zone while minimizing damage to cells not in the tissue zone, the voltage regulator is configured to use a Laplace equation to calculate the electrical potential distribution in tissue during typical electroporation pulses and is further configured to use a modified Pennes equation to calculate the resulting temperature distribution.

2. The device of claim 1, wherein the voltage regulator provides pulses of 100 microseconds±

about 10 microseconds at a voltage gradient in a range of from about 50 volt/cm to about 8000 volt/cm.

3. The system of claim 1, wherein the voltage regulator provides pulses at a voltage gradient of at least 600 v/cm.