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Differentiation and expansion of endothelial cells from pluripotent stem cells and the in vitro formation of vasculature like structures

  • US 10,119,122 B2
  • Filed: 06/10/2014
  • Issued: 11/06/2018
  • Est. Priority Date: 06/10/2013
  • Status: Active Grant
First Claim
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1. A method for producing an in vitro cell culture comprising endothelial cells derived from in vitro differentiated pluripotent stein cells and smooth muscle cells derived from in vitro differentiated pluripotent stem cells or derived from pericytes, the method comprising the following steps:

  • (a) culturing pluripotent stem cells in defined medium comprising ActivinA, BMP4, VEGF, and a canonical WNT ligand or GSK3 inhibitor, to produce a culture comprising differentiated cells;

    (b) culturing the cells obtained in step (a) in defined medium containing VEGF and a TGF{beta} signaling inhibitor to produce endothelial cells; and

    (c) culturing the endothelial cells obtained in step (b) with smooth muscle cells derived from in vitro differentiated pluripotent stem cells or derived from pericytes.

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