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Modified RNAi polynucleotides and uses thereof

  • US 10,131,904 B2
  • Filed: 02/11/2009
  • Issued: 11/20/2018
  • Est. Priority Date: 02/11/2008
  • Status: Active Grant
First Claim
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1. A blunt-ended double-stranded RNA (dsRNA) construct, for inhibiting expression of a target gene, comprising a sense strand of 25, 26, 27, 28, 29, or 30 nucleotides in length, having a 5′

  • -end and a 3′

    -end, wherein the sense strand consists of 2′

    -modified ribose sugars except for nucleotides 11, 12, and 13 relative to the 3′

    -end of the sense strand, which prevents processing of the double-stranded RNA construct by Dicer, and an antisense strand of 25, 26, 27, 28, 29, or 30 nucleotides in length having a 5′

    -end and a 3′

    -end, wherein the antisense strand is unmodified, wherein the antisense strand hybridizes to the sense strand and to mRNA of the target gene, wherein the dsRNA inhibits expression of the target gene in a sequence-dependent manner, and wherein the dsRNA does not form a hairpin.

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