DDAO compounds as fluorescent reference standards
First Claim
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1. A kit for amplification comprising:
- a buffer,a selection of nucleotides,a reference dye, andinstructions for carrying out an assay;
wherein the reference dye has a structure according to formula (I);
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Abstract
According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I):
In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.
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Citations
12 Claims
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1. A kit for amplification comprising:
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a buffer, a selection of nucleotides, a reference dye, and instructions for carrying out an assay; wherein the reference dye has a structure according to formula (I); - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification