×

Method for measuring the plasma concentration of an analyte directly on a whole blood sample

  • US 10,132,800 B2
  • Filed: 05/12/2014
  • Issued: 11/20/2018
  • Est. Priority Date: 05/13/2013
  • Status: Active Grant
First Claim
Patent Images

1. A method of measuring an amount of analyte in a whole blood sample, wherein the method comprises:

  • performing a calibration of the method,wherein the calibration comprises;

    providing a plurality of calibration whole blood samples;

    measuring an haematocrit level and an analyte amount directly in each of the plurality of calibration whole blood samples;

    measuring an analyte amount in a plasma sample from each of the plurality of calibration whole blood samples; and

    calculating polynomial coefficients of relation DP=Pa(DST, DH) from values of haematocrit level and analyte amount measured in the plurality of calibration whole blood samples, and values of analyte amount measured in the plasma samples from the plurality of calibration whole blood samples,where DP is the measured analyte amount in plasma, DST is the measured analyte amount in whole blood, DH is the measured haematocrit level, and Pa is a non-constant polynomial of a degree greater than or equal to 1 having as indeterminate values the measured analyte amount, DST, and the measured haematocrit level, DH, said relation having polynomial coefficients depending on the analyte;

    measuring an analyte amount directly in the whole blood sample;

    calculating a corrected analyte amount according to the relation;


    DP=Pa(DST, DH)where DP is the corrected analyte amount, DSTis the measured analyte amount, and DH is the measured haematocrit level Pa DST DH; and

    providing the calculated value of the corrected analyte amount.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×