Irradiation of red blood cells and anaerobic storage

• US 10,136,635 B2
• Filed: 11/04/2011
• Issued: 11/27/2018
• Est. Priority Date: 05/05/2010
• Status: Active Grant

• Current Assignee: Hemanext Inc.
• Original Assignee: New Health Sciences, Inc.
• Inventors: Yoshida, Tatsuro, Vernucci, Paul J.
• Primary Examiner(s): Zimbouski, Ariana
• Application Number:
  US13/289,722
• Publication Number:
  US 20120115124A1
• Time in Patent Office: 2,580 Days
• Field of Search: 604408
• CPC Class Codes: A01N 1/0205 Chemical aspects
• CPC Class Codes: A01N 1/0242 Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
• CPC Class Codes: A01N 1/0263 Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
• CPC Class Codes: A01N 1/0278 Physical preservation processes
• CPC Class Codes: A01N 1/0294 Electromagnetic, i.e. using electromagnetic radiation or electromagnetic fields
• CPC Class Codes: A61J 1/10 Bag-type containers
• CPC Class Codes: A61K 35/18 Erythrocytes haemoglobin A61K38/42
• CPC Class Codes: A61L 2/081 Gamma radiation
• CPC Class Codes: A61L 2/082 X-rays
• CPC Class Codes: A61L 2202/181 Flexible packaging means, e.g. permeable membranes, paper
• CPC Class Codes: A61L 2202/22 Blood or products thereof

Abstract

A blood storage system comprising: a collection vessel for red blood cells; an oxygen or oxygen and carbon dioxide depletion device; a storage vessel for red blood cells; tubing connecting the collection vessel to the oxygen or oxygen and carbon dioxide depletion device and the oxygen or oxygen and carbon dioxide depletion device to the storage vessel; and a gamma or X-ray irradiating device is used to irradiate red blood cells stored in the vessel, storing red blood cells under anaerobic conditions.

Claims

1. A blood storage system for reducing irradiation damage to red blood cells comprising:

a collection vessel for red blood cells;

an oxygen or oxygen and carbon dioxide depletion device comprising a cartridge;

a plurality of hollow fibers or gas-permeable films extending within the cartridge from an entrance to an exit thereof, wherein the hollow fibers or gas-permeable films are adapted to receiving and conveying red blood cells; and

an oxygen and carbon dioxide scavenger packed within the cartridge and contiguous to and in between the plurality of hollow fibers or gas-permeable films;

a storage vessel for storing oxygen or oxygen and carbon dioxide-depleted red blood cells under an anaerobic condition;

a first tubing connecting the collection vessel to the oxygen or oxygen and carbon dioxide depletion device; and

a second tubing connecting the oxygen or oxygen and carbon dioxide depletion device to the storage vessel;

wherein said oxygen or oxygen and carbon dioxide-depleted red blood cells are capable of being irradiated with a gamma- or X-ray irradiating device, when stored in said storage vessel; and

wherein said irradiation damage to said oxygen and carbon dioxide-depleted red blood cells is reduced without the addition of L-carnitine or an alkanoul derivative to said blood storage system and said oxygen and carbon dioxide-depleted red blood cells.

2. The blood storage system according to claim 1, wherein said anaerobic condition is measured as an oxygen-saturation (SO₂) of hemoglobin in said red blood cells of less than 20% SO₂.

3. The blood storage system according to claim 2, wherein said oxygen-saturation of hemoglobin in said red blood cells is less than 5% SO₂.

4. The blood storage system according to claim 3, wherein said oxygen-saturation of hemoglobin in said red blood cells is less than 3% SO₂.

5. The blood storage system according to claim 1, wherein said oxygen or oxygen and carbon dioxide depletion device is disposable.

6. The blood storage system according to claim 1, further comprising a leuko reduction filter disposed between said collection vessel and said oxygen or oxygen and carbon dioxide depletion device.

7. The blood storage system according to claim 1, further comprising an additive solution vessel in communication with said collection vessel.

8. The blood storage system according to claim 1, further comprising a plasma vessel in communication with said collection vessel.

9. The blood storage system according to claim 1, wherein said anaerobic condition is measured as a partial pressure of oxygen (PO₂) of less than 0.08 mmHg.

10. The blood storage system according to claim 1, wherein said storage vessel is substantially impermeable to oxygen or oxygen and carbon dioxide.

11. The blood storage system according to claim 1, wherein said storage vessel comprises an inner layer compatible with blood and an outer layer substantially impermeable to oxygen or oxygen and carbon dioxide.