Reducing the proliferation of adenocarcinoma cells (CaCo-2) by administration of a poly-oxygenated metal hydroxide

• US 10,137,148 B2
• Filed: 05/18/2018
• Issued: 11/27/2018
• Est. Priority Date: 03/30/2016
• Status: Active Grant

• Current Assignee: Baylor University
• Original Assignee: Baylor University
• Inventors: Bruce, Erica D., Woodmansee, Jr., John W.
• Primary Examiner(s): Arnold, Ernst V
• Assistant Examiner(s): Chang, Kyung S
• Application Number:
  US15/984,035
• Publication Number:
  US 20180264035A1
• Time in Patent Office: 193 Days
• Field of Search: None
• CPC Class Codes: A61K 33/06 Aluminium, calcium or magnesium; Compounds thereof , e.g. clay
• CPC Class Codes: A61K 33/08 Oxides; Hydroxides
• CPC Class Codes: A61K 47/02 Inorganic compounds
• CPC Class Codes: A61K 47/52 the modifying agent being an inorganic compound, e.g. an inorganic ion that is complexed with the active ingredient
• CPC Class Codes: A61K 47/6921 the form being a particulate, a powder, an adsorbate, a bead or a sphere
• CPC Class Codes: A61K 47/6923 the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
• CPC Class Codes: A61K 9/0026 Blood substitute; Oxygen transporting formulations; Plasma extender
• CPC Class Codes: A61K 9/08 Solutions composition of solutions A61K47/00

Abstract

A method of treating a mammal, comprising administering a therapeutically effective amount of a poly-oxygenated metal hydroxide composition to a mammal to reduce a proliferation of hypoxic carcinoma cells, wherein the poly-oxygenated metal hydroxide composition comprises a clathrate containing free oxygen gas (O₂) molecules. The carcinoma cells may comprise colon adenocarcinoma cells (CaCo-2). The poly-oxygenated metal hydroxide material is configured to provide bioavailable oxygen gas molecules to a mammal when administered to the mammal. The poly-oxygenated metal hydroxide composition can be administered intravenously, directly to carcinoma cells, and orally. The composition may comprise a fluid, where the poly-oxygenated metal hydroxide composition is soluble in the fluid.

Claims

1. A method of treating a mammal, comprising administering a therapeutically effective amount of a poly-oxygenated aluminum hydroxide composition to the mammal in need thereof to reduce a proliferation of colon adenocarcinoma CaCo-2 cells, wherein the poly-oxygenated aluminum hydroxide composition comprises a clathrate containing fee oxygen gas (O2) molecules;

and wherein the therapeutically effective amount of the poly-oxygenated aluminum hydroxide composition ranges from 10 mg/L to 1000 mg/L.

2. The method as specified in claim 1 wherein the poly-oxygenated aluminum hydroxide material is configured to provide bioavailable oxygen gas molecule to the mammal when administered to the mammal.

3. The method as specified in claim 1 wherein the poly-oxygenated aluminum hydroxide composition is administered intravenously.

4. The method as specified in claim 1 wherein the poly-oxygenated aluminum hydroxide composition is administered direct to the colon adenocarcinoma CaCo-2 cells.

5. The method as specified in claim 1 where the poly-oxygenated aluminum hydroxide composition is administered orally.

6. The method as specified in claim 1 wherein the compsition comprises a fluid.

7. The method as specified in claim 1 wherein the poly-oxygenated aluminum hydroxide composition soluble in the fluid.

8. The method as specified claim 6 wherein the fluid is a phosphate buffered saline (PBS).

9. The method as specified in claim 6 wherein the fluid is lactated ringers solution (LRS).

10. The method as specified in claim 1 wherein the poly-oxygenated aluminum hydroxide composition comprises poly-oxygenated aluminum hydroxide particles all having a diameter of less than or equal to 10 um.

11. The method as specified in claim 10 wherein the polyoxygenated aluminum particles are homogeneous.

12. The method as specified in claim 1 wherein the poly-oxygenated aluminum hydroxide composition comprises poly-oxygenated aluminum hydroxide particles all having a diameter of less than or equal to 1 um.

13. The method as specified in claim 1 wherein the poly-oxygenated aluminum hydroxide composition comprises poly-oxygenated aluminum hydroxide particles that are surface modified.

14. The method as specified claim 13 wherein the poly-oxygenated aluminum hydroxide material comprises particles that are surface modified with polyethylene glycol (PEG).

15. The method as specified in claim 1 wherein the mammal is a human individual.