Tissue processing apparatus and method for processing adipose tissue
First Claim
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1. A method for processing biological material containing stringy tissue, the method comprising;
- providing a container havingat least one inlet port,an internal containment volume, the internal containment volume including a tissue retention volume and a filtrate volume separated from the tissue retention volume by a filter, anda tissue collector disposed in the tissue retention volume and rotatable relative to the container in a direction of rotation about an axis of rotation, the tissue collector including multiple teeth positioned on the tissue collector;
washing a biological material contained in the tissue retention volume by adding a wash liquid to the tissue retention volume and allowing the wash liquid and contaminants to pass through the filter from the tissue retention volume to the filtrate volume; and
rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector.
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Abstract
A method for processing biological material containing stringy tissue in a container having a tissue collector disposed in a tissue retention volume on one side of an internal filter includes washing biological material contained in the tissue retention volume with wash liquid to the tissue retention volume and allowing the wash liquid and rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector.
115 Citations
20 Claims
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1. A method for processing biological material containing stringy tissue, the method comprising;
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providing a container having at least one inlet port, an internal containment volume, the internal containment volume including a tissue retention volume and a filtrate volume separated from the tissue retention volume by a filter, and a tissue collector disposed in the tissue retention volume and rotatable relative to the container in a direction of rotation about an axis of rotation, the tissue collector including multiple teeth positioned on the tissue collector; washing a biological material contained in the tissue retention volume by adding a wash liquid to the tissue retention volume and allowing the wash liquid and contaminants to pass through the filter from the tissue retention volume to the filtrate volume; and rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect stringy material on the tissue collector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for processing biological material containing stringy tissue, the method comprising;
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providing a container having at least one inlet port, an internal containment volume, the internal containment volume including a tissue retention volume and a filtrate volume separated from the tissue retention volume by a filter, and a tissue collector disposed in the tissue retention volume and rotatable relative to the container in a direction of rotation about an axis of rotation, the tissue collector including multiple teeth positioned on the tissue collector; delivering biological material containing adipose and stringy material through the at least one inlet port into the tissue retention volume; and rotating the tissue collector disposed in the tissue retention volume relative to the container in a first direction of rotation about an axis of rotation to sweep the teeth positioned on the tissue collector through the biological material and to collect the stringy material on the tissue collector. - View Dependent Claims (14, 15, 16, 17, 18, 19)
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20. A method for processing biological material containing stringy tissue, the method comprising;
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providing a container having at least one inlet port, an internal containment volume, the internal containment volume including a tissue retention volume and a filtrate volume separated from the tissue retention volume by a filter, a tissue collector disposed in the tissue retention volume and rotatable relative to the container in a direction of rotation about an axis of rotation, the tissue collector including multiple teeth positioned on the tissue collector, and a mixing propeller disposed in the tissue retention volume and rotatable relative to the container in a direction of rotation about an axis of rotation; and rotating the mixing propeller and the tissue collector disposed in the tissue retention volume relative to the container about an axis of rotation to mix a biological material contained in the tissue retention volume and to move the teeth positioned on the tissue collector through the biological material and to collect the stringy material on the tissue collector.
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Specification