Metabolic analyzer
First Claim
1. A method for metabolic analysis of a subject comprising:
- providing a solid support or porous membrane including a plurality of sensing binding sites;
selecting an array of sensing materials where each sensing material is capable of reacting with a specific analyte so that when operating together at least two different metabolic signatures can be sensed and wherein each sensing material changes absorbance upon interactions with its specific analyte;
affixing the array of sensing materials to the plurality of sensing binding sites;
affixing at least one reference area to the plurality of sensing binding sites, where the at least one reference area is insensitive to the analytes to allow corrections and noise removal;
illuminating the plurality of sensing binding sites with at least one light source providing incident light;
operating at least one light detector for simultaneously collecting and detecting a first plurality of non-fluorescence initiated color change signals from the plurality of sensing binding sites at an absorbance wavelength, wherein the first plurality of non-fluorescence initiated color change signals consists of a first change of light intensity of the incident light transmitted through or reflected from the solid support, collected at the absorbance wavelength, and includes characteristic colors for oxygen, and carbon dioxide, and the at least one reference area;
introducing a flow of gases to the plurality of sensing binding sites;
operating the at least one light detector for simultaneously collecting and detecting a second plurality of non-fluorescence initiated color change signals from the plurality of sensing binding sites when exposed to the flow of gases, wherein the second plurality of non-fluorescence initiated color change signals consists of a second change of light intensity at the absorbance wavelength transmitted through or reflected from the solid support;
comparing the first plurality of non-fluorescence initiated color change signals to the second plurality of non-fluorescence initiated color change signals to generate a plurality of registered non-fluorescence initiated color changes;
processing the plurality of registered non-fluorescence initiated color changes to determine the changes in oxygen concentration and carbon dioxide concentration; and
determining the at least two different metabolic signatures from the changes in oxygen concentration and carbon dioxide concentration.
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Accused Products
Abstract
A method for weight and/or fitness management using a metabolic analyzer that measures metabolic data including oxygen and carbon dioxide. The metabolic analyzer includes integrated collection-detection sensors with for high efficiency and collection, high specificity and simultaneous detection of at least two metabolic signatures, including at least oxygen and carbon dioxide, in breath via a porous membrane with high density of sensing binding sites, where the porous membrane includes sensing materials such that the sensing binding sites are specific to the metabolic signatures, and change colors upon interactions with the metabolic signatures. Weight of the subject is measured using a weight measurement device and a recommendation for food intake and/or physical activity is based on at least the readings of the metabolic analyzer and weight of the subject.
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Citations
17 Claims
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1. A method for metabolic analysis of a subject comprising:
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providing a solid support or porous membrane including a plurality of sensing binding sites; selecting an array of sensing materials where each sensing material is capable of reacting with a specific analyte so that when operating together at least two different metabolic signatures can be sensed and wherein each sensing material changes absorbance upon interactions with its specific analyte; affixing the array of sensing materials to the plurality of sensing binding sites; affixing at least one reference area to the plurality of sensing binding sites, where the at least one reference area is insensitive to the analytes to allow corrections and noise removal; illuminating the plurality of sensing binding sites with at least one light source providing incident light; operating at least one light detector for simultaneously collecting and detecting a first plurality of non-fluorescence initiated color change signals from the plurality of sensing binding sites at an absorbance wavelength, wherein the first plurality of non-fluorescence initiated color change signals consists of a first change of light intensity of the incident light transmitted through or reflected from the solid support, collected at the absorbance wavelength, and includes characteristic colors for oxygen, and carbon dioxide, and the at least one reference area; introducing a flow of gases to the plurality of sensing binding sites; operating the at least one light detector for simultaneously collecting and detecting a second plurality of non-fluorescence initiated color change signals from the plurality of sensing binding sites when exposed to the flow of gases, wherein the second plurality of non-fluorescence initiated color change signals consists of a second change of light intensity at the absorbance wavelength transmitted through or reflected from the solid support; comparing the first plurality of non-fluorescence initiated color change signals to the second plurality of non-fluorescence initiated color change signals to generate a plurality of registered non-fluorescence initiated color changes; processing the plurality of registered non-fluorescence initiated color changes to determine the changes in oxygen concentration and carbon dioxide concentration; and determining the at least two different metabolic signatures from the changes in oxygen concentration and carbon dioxide concentration. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification