Metabolic analyzer

• US 10,143,401 B2
• Filed: 06/11/2012
• Issued: 12/04/2018
• Est. Priority Date: 06/13/2011
• Status: Active Grant

• Current Assignee: Arizona Board of Regents (University of Arizona)
• Original Assignee: Arizona Board of Regents (University of Arizona)
• Inventors: Tao, Nongjian, Forzani, Erica
• Primary Examiner(s): Agahi, Puya
• Application Number:
  US13/493,552
• Publication Number:
  US 20130150746A1
• Time in Patent Office: 2,367 Days
• Field of Search: 600301, 600529, 600531, 600532, 600538, 600540, 600543, 128921
• CPC Class Codes: A61B 5/08 Detecting, measuring or recording devices for evaluating the respiratory organs A61B5/0205 takes precedence
• CPC Class Codes: A61B 5/083 Measuring rate of metabolism by using breath test, e.g. measuring rate of oxygen consumption
• CPC Class Codes: A61B 5/0833 Measuring rate of oxygen consumption
• CPC Class Codes: A61B 5/0836 Measuring rate of CO2 production
• CPC Class Codes: G16H 10/40 for data related to laboratory analysis, e.g. patient specimen analysis
• CPC Class Codes: Y02P 90/84 Greenhouse gas [GHG] management systems
• CPC Class Codes: Y02P 90/845 Inventory and reporting systems for greenhouse gases [GHG]

Abstract

A method for weight and/or fitness management using a metabolic analyzer that measures metabolic data including oxygen and carbon dioxide. The metabolic analyzer includes integrated collection-detection sensors with for high efficiency and collection, high specificity and simultaneous detection of at least two metabolic signatures, including at least oxygen and carbon dioxide, in breath via a porous membrane with high density of sensing binding sites, where the porous membrane includes sensing materials such that the sensing binding sites are specific to the metabolic signatures, and change colors upon interactions with the metabolic signatures. Weight of the subject is measured using a weight measurement device and a recommendation for food intake and/or physical activity is based on at least the readings of the metabolic analyzer and weight of the subject.

Claims

1. A method for metabolic analysis of a subject comprising:

providing a solid support or porous membrane including a plurality of sensing binding sites;

selecting an array of sensing materials where each sensing material is capable of reacting with a specific analyte so that when operating together at least two different metabolic signatures can be sensed and wherein each sensing material changes absorbance upon interactions with its specific analyte;

affixing the array of sensing materials to the plurality of sensing binding sites;

affixing at least one reference area to the plurality of sensing binding sites, where the at least one reference area is insensitive to the analytes to allow corrections and noise removal;

illuminating the plurality of sensing binding sites with at least one light source providing incident light;

operating at least one light detector for simultaneously collecting and detecting a first plurality of non-fluorescence initiated color change signals from the plurality of sensing binding sites at an absorbance wavelength, wherein the first plurality of non-fluorescence initiated color change signals consists of a first change of light intensity of the incident light transmitted through or reflected from the solid support, collected at the absorbance wavelength, and includes characteristic colors for oxygen, and carbon dioxide, and the at least one reference area;

introducing a flow of gases to the plurality of sensing binding sites;

operating the at least one light detector for simultaneously collecting and detecting a second plurality of non-fluorescence initiated color change signals from the plurality of sensing binding sites when exposed to the flow of gases, wherein the second plurality of non-fluorescence initiated color change signals consists of a second change of light intensity at the absorbance wavelength transmitted through or reflected from the solid support;

comparing the first plurality of non-fluorescence initiated color change signals to the second plurality of non-fluorescence initiated color change signals to generate a plurality of registered non-fluorescence initiated color changes;

processing the plurality of registered non-fluorescence initiated color changes to determine the changes in oxygen concentration and carbon dioxide concentration; and

determining the at least two different metabolic signatures from the changes in oxygen concentration and carbon dioxide concentration.

2. The method of claim 1 further comprising processing the at least two different metabolic signatures for measuring resting energy expenditure (REE), and respiratory quotient (RQ).

3. The method of claim 2 wherein recommendations for diet and exercise goals are produced on changes of REE and RQ.

4. The method of claim 1 further comprising processing the at least two different metabolic signatures to measure ketones, sulfur compounds, ammonia and water.

5. The method of claim 1 further comprising:

wherein the act of introducing a flow of gases to the plurality of sensing binding sites consists of introducing breath to flow over the array of sensing materials through a mouthpiece attached to the solid support or porous membrane; and

monitoring breath volume or rate from the mouthpiece.

6. The method of claim 1 wherein the at least one light detector includes a photodetector, complementary metal oxide semiconductor (CMOS), or a charge-coupled device (CCD).

7. The method of claim 5 wherein monitoring breath volume or rate comprises operating a flow meter or a pressure sensor from which the breath volume or rate is determined.

8. The method of claim 5 wherein monitoring breath volume or rate comprises coupling a bag with a fixed volume to an outlet of the mouthpiece to collect a breath after passing it over the sensing materials in a timed period.

9. The method of claim 1 further comprising operating a mobile device to carry out the acts of comparing the first plurality of color change signals to the second plurality of color change signals to generate a plurality of registered color changes, determining the change in oxygen concentration and carbon dioxide concentration responsively to the plurality of registered color changes;

and determining the at least two different metabolic signatures from the change in oxygen concentration and carbon dioxide concentration.

10. The method of claim 5 further comprising locating the plurality of sensing binding sites on the mouthpiece.

11. The method of claim 5 further comprising locating the at least one light source and the at least one light sensor at opposite sides of the array of sensing materials.

12. The method of claim 5 further comprising locating the at least one light source and the at least one light sensor on the same side relative to the solid support or porous membrane.

13. The method of claim 5 further comprising wirelessly transmitting signals from the at least one light sensor.

14. The method of claim 5 further comprising integrating the mouthpiece, the at least one light source, the array of sensing materials and the at least one light sensor into a unitary device.

15. The method of claim 1 wherein the array of sensing materials comprises nanoparticles or micro particles.

16. The method of claim 1 wherein the solid support comprises a material selected from a group consisting of synthetic polymers, natural polymers, polyester, nylon, cellulose, glass-produced substrates, fiber glass, sol gel, silica, alumina, silica gel and composites of the materials listed in the group.

17. The method of claim 14 further comprising monitoring metabolic processes including:

integrating the unitary device in a cell phone; and

using the unitary device as a metabolic analyzer for detecting consumed oxygen rate and produced carbon dioxide rate from breath obtained with a fixed volume in a timed period.