Magnetic immunosensor and method of use
First Claim
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1. A magnetic immunosensing device, comprising:
- a sensing electrode on a substantially planar chip, wherein the electrode is positioned in a conduit for receiving a sample mixed with antibody-labeled magnetically susceptible beads; and
a microfabricated magnetic layer, wherein the microfabricated magnetic layer comprises high-field permanent magnetic particles dispersed in a thermally, chemically, or photoformably cured immobilization matrix, wherein the immobilization matrix comprises a polyimide, polyvinyl alcohol, or epoxy resin, wherein the magnetic layer is positioned relative to the electrode, thereby attracting the beads substantially proximate to the electrode and substantially retaining the beads at the electrode surface during removal of unbound sample and washing of the electrode.
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Abstract
The present invention provides apparatus and methods for the rapid determination of analytes in liquid samples by immunoassays incorporating magnetic capture of beads on a sensor capable of being used in the point-of-care diagnostic field.
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Citations
20 Claims
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1. A magnetic immunosensing device, comprising:
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a sensing electrode on a substantially planar chip, wherein the electrode is positioned in a conduit for receiving a sample mixed with antibody-labeled magnetically susceptible beads; and a microfabricated magnetic layer, wherein the microfabricated magnetic layer comprises high-field permanent magnetic particles dispersed in a thermally, chemically, or photoformably cured immobilization matrix, wherein the immobilization matrix comprises a polyimide, polyvinyl alcohol, or epoxy resin, wherein the magnetic layer is positioned relative to the electrode, thereby attracting the beads substantially proximate to the electrode and substantially retaining the beads at the electrode surface during removal of unbound sample and washing of the electrode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification