Method for preparing an artificial heart valve leaflet

• US 10,149,755 B2
• Filed: 06/29/2016
• Issued: 12/11/2018
• Est. Priority Date: 12/31/2013
• Status: Active Grant

• Current Assignee: Kingstronbio (Changshu) Co., Ltd.
• Original Assignee: Kingstronbio (Changshu) Co., Ltd.
• Inventors: Zhong, Shengping Sam, Jin, Chang, Liu, Jing
• Primary Examiner(s): Wu, Julie
• Assistant Examiner(s): White, Douglas F
• Application Number:
  US15/197,676
• Publication Number:
  US 20160310266A1
• Time in Patent Office: 895 Days
• Field of Search: None
• CPC Class Codes: A61F 2/2415 Manufacturing methods
• CPC Class Codes: A61F 2240/004 Using a positive or negative model, e.g. moulds
• CPC Class Codes: A61L 2430/20 for reconstruction of the heart, e.g. heart valves
• CPC Class Codes: A61L 27/367 Muscle tissue, e.g. sphincter
• CPC Class Codes: A61L 27/3687 characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
• CPC Class Codes: A61L 27/3691 characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062

Abstract

The present invention provides a method for preparing an artificial heart valve leaflet, comprising freezing an animal tissue membrane and processing the frozen animal tissue membrane so as to reach a preset thickness. The method, by processing the frozen animal tissue membrane, can conveniently obtain an artificial heart valve leaflet with a required thickness and increase the yield of the animal tissue membrane valve leaflet.

Claims

1. A method for preparing an artificial heart valve leaflet comprising

pre-treating an animal tissue membrane comprised of immersing the animal tissue membrane in a cryoprotectant,

freezing the animal tissue membrane comprised of placing the animal tissue membrane on a mould having a center that is convex and periphery that is concave relative to the center of said mould, and having a thickness distribution corresponding to that of the artificial heart valve leaflet;

and then together with the mould the animal tissue membrane is immersed into liquid nitrogen for freezing, and

processing the frozen animal tissue membrane to a preset thickness.

2. The method of claim 1, wherein the animal tissue membrane is immersed into the liquid nitrogen for processing.

3. The method of claim 1, wherein the processing comprises one or more of grinding, cutting, scraping, milling and ablation.

4. The method of claim 1, further comprising processing the animal tissue membrane to a required shape of the artificial heart valve leaflet, prior to freezing the animal tissue membrane, or after processing the frozen animal tissue membrane to the preset thickness.

5. The method of claim 1, wherein the processing is conducted on a fibrous surface of the animal tissue membrane.

6. The method of claim 1, wherein the animal tissue membrane is processed with a cross-linking treatment.

7. The method of claim 1, wherein the processing the frozen animal tissue membrane to a preset thickness comprises reducing the entire thickness of the animal tissue membrane to a preset thickness, or processing different portions of the animal tissue membrane to respective thicknesses as required.

8. The method of claim 1, wherein the processing the frozen animal tissue membrane to a preset thickness comprises placing the animal tissue membrane into a concave die with a depth equal to the preset thickness for processing, so as to reduce the entire thickness of the animal tissue membrane to the preset thickness.

9. The method of claim 1, wherein the animal tissue membrane is pericardium, diaphragm or endocranium of a mammal.

10. The method of claim 9, wherein the processing is conducted on a fibrous surface of the animal tissue membrane.