Massively Parallel single cell analysis
First Claim
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1. A method comprising:
- introducing a single bead with a single cell into a partition, wherein said single bead comprises a plurality of oligonucleotides, wherein each of said plurality of oligonucleotides comprises an identical cellular label sequence, a molecular label sequence, and a target-binding region, wherein the cellular label sequence comprises at least 6 nucleotides, wherein the molecular label sequence comprises at least 6 nucleotides, and wherein at least 100 of said plurality of oligonucleotides comprise different molecular label sequences.
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Abstract
The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
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Citations
30 Claims
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1. A method comprising:
- introducing a single bead with a single cell into a partition, wherein said single bead comprises a plurality of oligonucleotides, wherein each of said plurality of oligonucleotides comprises an identical cellular label sequence, a molecular label sequence, and a target-binding region, wherein the cellular label sequence comprises at least 6 nucleotides, wherein the molecular label sequence comprises at least 6 nucleotides, and wherein at least 100 of said plurality of oligonucleotides comprise different molecular label sequences.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
Specification