Magnetic and/or electric label assisted detection system and method

• US 10,151,750 B2
• Filed: 12/15/2014
• Issued: 12/11/2018
• Est. Priority Date: 10/12/2006
• Status: Active Grant

• Current Assignee: Siemens Healthcare Nederland B.V. (Siemens AG)
• Original Assignee: Koninklijke Philips N.V.
• Inventors: Dittmer, Wendy Uyen, Prims, Menno Willem Jose
• Primary Examiner(s): Brown, Melanie Yu
• Assistant Examiner(s): Moerschell, Richard
• Application Number:
  US14/570,381
• Publication Number:
  US 20150104784A1
• Time in Patent Office: 1,457 Days
• Field of Search: None
• CPC Class Codes: G01N 27/745 for detecting magnetic beads used in biochemical assays concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

A detection method (200) detects analytes in a fluid sample. The detection method includes transporting magnetic and/or electric labels (5) after interaction between the sample fluid and a reagent towards a detection receptacle (1). The detection receptacle (1) is initially substantially magnetic and/or electric label (5) free. By transporting the magnetic and/or electric labels (5) after reaction, interference between unreacted reagents and magnetic and/or electric label-assisted detection can be reduced.

Claims

1. A method for detecting at least one target molecule, the method comprising:

allowing interaction between a sample containing at least one target molecule and at least one primary magnetic and/or electric label to thereby obtain at least one labeled target molecule,

applying a first magnetic field having a first orientation to transport the at least one primary magnetic and/or electric labeled target molecule to a detection surface wherein the primary magnetic and/or electric labeled target molecule binds the detection surface,

applying a second magnetic field having a second orientation to transport at least one secondary magnetic and/or electric label to the detection surface,

allowing the at least one secondary magnetic and/or electric label to interact with the at least one primary magnetic or electric label to obtain at least one primary and secondary magnetic and/or electric labeled target module,

after said allowing of said interaction of the primary magnetic and/or electric labeled target molecule, detecting the at least one primary and secondary magnetic and/or electric label bound to the detection surface.

2. The method of claim 1, wherein the magnetic and/or electric label is a magnetic label.

3. The method according to claim 1, wherein the first and second orientations are substantially orthogonal to each other.

4. The method according to claim 1, wherein the first magnetic field transports the primary magnetic and/or electric label along the first orientation without transporting the secondary magnetic and/or electric label, and

the second magnetic field transports the secondary magnetic and/or electric label along a second orientation without transporting the primary magnetic and/or electric label.

5. The method according to claim 1, further including:

waiting until the primary magnetic and/or electric label is bound to the detector surface and then applying the first magnetic field again in a manner to transport unbound primary magnetic and/or electric labels out of the first receptacle.

6. The method of claim 1, wherein the detection surface is disposed in a first receptacle, the primary magnetic and/or electric label is contained in a second receptacle, and the secondary magnetic and/or electric label is contained in a third receptacle.

7. The method of claim 6, wherein the sample interacts with the primary magnetic and/or electric label in the first receptacle, and after a predetermined time, the secondary magnetic and/or electric label is transported to the first receptacle to react with the primary magnetic and/or electric label.

8. The method of claim 6, wherein the sample interacts with a reagent and the secondary magnetic and/or electric label in the second receptacle.

9. The method of claim 6, further comprising detecting a time of arrival in the first receptacle of a fluid containing the at least one target molecule.