Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices

• US 10,156,578 B2
• Filed: 12/21/2015
• Issued: 12/18/2018
• Est. Priority Date: 08/19/2004
• Status: Expired due to Fees

• Current Assignee: Blood Cell Storage, Inc.
• Original Assignee: Blood Cell Storage, Inc.
• Inventors: Reed, Michael W., Geelhood, Steven J., Harris, Paul C., Barker, Lynn M., Hargrave, Perry, Nanassy, Oliver Z.
• Primary Examiner(s): Bass, Dirk R
• Application Number:
  US14/977,350
• Publication Number:
  US 20160209432A1
• Time in Patent Office: 1,093 Days
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/22 Testing for sterility conditions
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 2201/0826 Fibre array at source, distributing
• CPC Class Codes: G01N 2201/084 Fibres for remote transmission
• CPC Class Codes: G01N 2400/00 Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
• CPC Class Codes: G01N 33/492 Determining multiple analytes
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

System and method for detecting and measuring chemical perturbations in a sample. The system and method are useful for non-invasive pH monitoring of blood or blood products sealed in storage bags.

Claims

1. A method for monitoring a parameter of one or more samples positioned in an incubator, comprising:

(a) irradiating a fluorescent species having a first emission intensity at a first emission wavelength and a second emission intensity at a second emission wavelength, wherein the first and second emission wavelengths are not the same, each emission intensity dependent on a parameter, with excitation light emanating from a probe physically isolated from the fluorescent species to provide first and second emission intensities, wherein the fluorescent species is in a sample contained in a vessel in an incubator;

(b) measuring the first and second emission intensities to determine a first parameter reading of the sample; and

(c) repeating step (a) after a pre-determined time and measuring the first and second emission intensities to determine a second parameter reading of the sample.

2. The method of claim 1, wherein step (c) is repeated to provide multiple parameter readings to monitor the parameter of the sample over time.

3. The method of claim 1, wherein the parameter is pH.

4. The method of claim 1, wherein the parameter is CO₂.

5. The method of claim 1, wherein the parameter is oxygen.

6. The method of claim 1, wherein the parameter is glucose.

7. The method of claim 1 further comprising measuring the first and second emission intensities of each sample positioned in the incubator to determine a first parameter reading for each of the samples.

8. The method of claim 7, wherein step (c) is repeated to provide multiple parameter readings to monitor the parameter of each of the samples over time.

9. A method for monitoring the pH of one or more samples positioned in an incubator, comprising:

(a) irradiating a fluorescent species in a sample contained in a vessel with excitation light emanating from a probe physically isolated from the fluorescent species, wherein the excitation light has a wavelength sufficient to effect fluorescent emission from the fluorescent species, wherein the fluorescent species exhibits a first emission intensity at a first emission wavelength and a second emission intensity at a second emission wavelength, the ratio of the first and second emission intensities being dependent on pH, wherein the first and second emission wavelengths are not the same;

(b) measuring the first and second emission intensities to determine the first pH of the sample in the incubator; and

(c) repeating step (a) after a pre-determined time and measuring the first and second emission intensities to determine the second pH of the sample in the incubator.

10. The method of claim 9, wherein step (c) is repeated to provide multiple pH readings to monitor the pH of the sample over time.

11. The method of claim 9 further comprising measuring the first and second emission intensities of each sample positioned in the incubator to determine a first parameter reading for each of the samples.

12. The method of claim 9, wherein step (c) is repeated to provide multiple parameter readings to monitor the parameter of each of the samples over time.