Isolated bacterial strain of the genus burkholderia and pesticidal metabolites therefrom

• US 10,159,250 B2
• Filed: 06/24/2016
• Issued: 12/25/2018
• Est. Priority Date: 02/25/2010
• Status: Active Grant

• Current Assignee: Pro Farm Group, Inc. (Bioceres Crop Solutions Corp.)
• Original Assignee: Marrone Bio Innovations Inc. (Bioceres Crop Solutions Corp.)
• Inventors: Asolkar, Ratnakar, Koivunen, Marja, Marrone, Pamela, Kreylos, Ana-Lucia Cordova, Huang, Huazhang
• Primary Examiner(s): Paguio Frising, Michelle F.
• Application Number:
  US15/192,016
• Publication Number:
  US 20160330975A1
• Time in Patent Office: 914 Days
• Field of Search: None
• CPC Class Codes: A01N 43/00 Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds containing cyclic anhydrides, cyclic imides A01N37/00; containing compounds of the formula containing only one heterocyclic ring, wherein m>=1 and n>=0 and is unsubstituted or alkylsubstituted pyrrolidine, piperidine, morpholine, thiomorpholine, piperazine or a polymethyleneimine with four or more CH2 groups, A01N33/00 - A01N41/12; containing cyclopropane carboxylic acids or derivatives thereof, e.g. esters having heterocyclic rings, A01N53/00
• CPC Class Codes: A01N 43/16 with oxygen as the ring hetero atom
• CPC Class Codes: A01N 43/74 five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
• CPC Class Codes: A01N 43/76 1,3-Oxazoles; Hydrogenated 1,3-oxazoles
• CPC Class Codes: A01N 43/86 six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
• CPC Class Codes: A01N 43/90 having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
• CPC Class Codes: A01N 57/20 containing acyclic or cycloaliphatic radicals
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: C07D 263/32 with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 263/34 with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 309/14 Nitrogen atoms not forming part of a nitro radical
• CPC Class Codes: C07D 407/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 413/04 directly linked by a ring-member-to-ring-member bond
• CPC Class Codes: C07D 413/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 493/10 Spiro-condensed systems
• CPC Class Codes: C07D 498/14 Ortho-condensed systems
• CPC Class Codes: C07D 513/04 Ortho-condensed systems
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 17/04 : containing a five-membered hetero ring, e.g. griseofulvin , vitamin C
• CPC Class Codes: C12P 17/06 : containing a six-membered hetero ring, e.g. fluorescein
• CPC Class Codes: C12P 17/14 : Nitrogen or oxygen as hetero atom and at least one other diverse hetero ring atom in the same ring
• CPC Class Codes: C12P 17/16 : containing two or more hetero rings thiamine open chain analogs C12P17/167, i.e. not condensed among themselves or through a common carbocyclic ring system
• CPC Class Codes: C12P 17/18 : containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin e.g. Rifamycin C12P17/189
• CPC Class Codes: C12R 2001/00 : Microorganisms ; Processes using microorganisms
• CPC Class Codes: C12R 2001/01 : Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Abstract

A species of Burkholderia sp with no known pathogenicity to vertebrates but with pesticidal activity (e.g., plants, insects, fungi, weeds and nematodes) is provided. Also provided are natural products derived from a culture of said species and methods of controlling pests using said natural products.

Claims

1. A method for inhibiting infestation of Rhagoletis spp., Drosophila spp., Delia spp., or Musca spp. in a location comprising

applying an effective amount of

a whole cell broth, filtrate, supernatant, or extract collected from Burkholderia strain A396 (NRRL Accession No. B-50319) fermentation, and

at least one of a carrier, diluent or adjuvant.

2. The method according to claim 1 wherein said Rhagoletis spp. is Rhagoletis completa or Rhagoletis pomonella.

3. The method according to claim 1, wherein said Rhagoletis spp., Drosophila spp., Delia spp., or Musca spp. is inhibited by increasing the mortality of said Rhagoletis spp., Drosophila spp., Delia spp., or Musca spp.

4. The method according to claim 1, which further comprises applying another natural or artificial insecticidal substance.

5. The method of claim 4, wherein said another natural or artificial insecticidal substance is applied at said location in rotation.

6. The method according to claim 1 wherein said Drosophila spp. is Drosophila suzuki.

7. The method according to claim 1 wherein said Delia spp. is Delia platura.

8. The method according to claim 1 wherein said Musca spp. is Musca domestica.