System and method of co-cultivating microalgae with fungus

• US 10,160,989 B2
• Filed: 01/09/2012
• Issued: 12/25/2018
• Est. Priority Date: 05/02/2011
• Status: Active Grant

• Current Assignee: Renewuel LLC
• Original Assignee: Renewuel LLC
• Inventors: Yuan, Joshua (Shuhua), Xie, Shangxian
• Primary Examiner(s): Claytor, Renee
• Assistant Examiner(s): Van Buren, Lauren K
• Application Number:
  US13/346,426
• Publication Number:
  US 20120282651A1
• Time in Patent Office: 2,542 Days
• Field of Search: None
• CPC Class Codes: C12N 1/12 Unicellular algae; Culture media therefor as new plants A01H13/00
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12P 39/00 Processes involving microorganisms of different genera in the same process, simultaneously
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil

Abstract

One of the most important technical barriers for algal biofuel is the substantial harvesting and extracting cost. The present invention includes a system and method for the pelletization of single cell microalgae through co-cultivation with filamentous fungi to enable the low cost separation of microalgae from liquid medium and to significantly increase the biomass and lipid yield. The approach represents a simple and straightforward method to significantly reduce the cost for algae-based biofuel production and enable the economically viable algae biofuel production.

Claims

1. A system for the production of a biological product, comprising:

at least one fungus;

at least one algae; and

a growth medium,

wherein the at least one fungus and at least one algae are co-cultivated in or on the growth medium to form a pellet in or on the growth medium during co-cultivation, such that at least 50% of the co-cultivated algae is contained within the pellet, and wherein the pellet contains the biological product.

2. The system of claim 1, wherein the at least one fungus is from a class selected from the group consisting of Arthoniomycetes, Agaricomycetes, Blastocladiomycetes, Chytridiomycetes, Dacrymycetes, Dothideomycetes, Geoglossomycetes, Glomeromycetes, Eurotiomycetes, Lecanoromycetes, Leotiomycetes, Lichinomycetes, Monoblepharidomycetes, Orbiliomycetes, Sordariomycetes, Tremellomycetes, Pezizomycetes, Zygomycetes, and combinations thereof.

3. The system of claim 1, wherein the at least one algae is from a class selected from the group consisting of Actinochrysophyceae, Bacillariophyceae, Bryopsidophyceae, Bolidophyceae, Chlorarachnea, Chlorophyceae, Chrysophyceae, Cryptophyceae, Cyanophyceae, Diatomophyceae, Dinophyceae, Eustigmatophyceae, Glaucophyceae, Haptophyceae, Noctiluciphyceae, Pedinophyceae, Picophagophyceae, Pleurastrophyceae, Prasinophyceae, Prymnesiophyceae, Raphidophyceae, Synchromophyceae, Syndiniophyceae, Synurophyceae, Trebouxiophyceae, Ulvophyceae, Xanthophyceae, and combinations thereof.

4. The system of claim 3, wherein the at least one algae is from a genus selected from the group consisting of Actinocyclus, Cyclotella, Cryptomonas, Monochrysis Chlorella, Chaetoceros, Haematococcus, Nannochloropsis, Pleurochrysis, Gracilaria, Sargassum, Dunaliella, Cyclotella, Navicula, Nitzschia, Spirulina, Phaeodactylum, Thalassiosira, Skeletonema, Porphyra and combinations thereof.

5. The system of claim 1, wherein the biological product is selected from the group consisting of a biofuel, a biomass, a terpenoid compound, a bioplastic, a pigment, an antioxidant, a vitamin, an antibiotic, a surfactant protein, a peptide, a lipid, a therapeutic compound and combinations thereof.

6. The system of claim 5, wherein the terpenoid compound is selected from the group consisting of squalene, limonene, botryococcene and combinations thereof.

7. The system of claim 5, wherein the therapeutic compound is a neutraceutical.

8. The system of claim 7, wherein the neutraceutical is an unsaturated fatty acid including DHA and EPA.

9. The system of claim 5, wherein the therapeutic compound is a protein, peptide or lipid.

10. The system of claim 1, wherein the ratio of fungus to algae is about 1 to 1.

11. The system of claim 1, wherein the ratio of fungus to algae is about 2 to 1.

12. The system of claim 1, wherein the ratio of fungus to algae is about 1 to 2.

13. The system of claim 1, wherein the growth medium is selected from the group consisting of waste water, lignocellulosic biomass, corn, waste from bioethanol, biofuel production, waste from industrial processes and combinations thereof.

14. The system of claim 1, wherein the pellet has a diameter ranging between 0.2-15 mm.