Matrices and media for storage and stabilization of biomolecules

• US 10,160,997 B2
• Filed: 02/07/2017
• Issued: 12/25/2018
• Est. Priority Date: 09/12/2008
• Status: Active Grant

• Current Assignee: GenTegra, LLC (Genisyss, LLC)
• Original Assignee: GenTegra, LLC (Genisyss, LLC)
• Inventors: Hogan, Michael, Saghbini, Michael, Wong, David
• Primary Examiner(s): Thomas, David C
• Application Number:
  US15/426,706
• Publication Number:
  US 20170145477A1
• Time in Patent Office: 686 Days
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C09K 15/30 containing heterocyclic ring with at least one nitrogen atom as ring member
• CPC Class Codes: C12N 15/10 Processes for the isolation, preparation or purification of DNA or RNA chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34
• CPC Class Codes: C12N 5/0632 Cells of the oral mucosa
• CPC Class Codes: C12N 5/0634 Cells from the blood or the immune system
• CPC Class Codes: C12N 9/96 Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: Y10T 428/139 Open-ended, self-supporting conduit, cylinder, or tube-type article
• CPC Class Codes: Y10T 428/1397 Single layer [continuous layer]

Abstract

The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.

Claims

1. An aqueous medium comprising:

boric acid or a corresponding salt of boric acid;

a plasticizer and a hydroxyl radical scavenger, wherein histidine is the plasticizer and the hydroxyl radical scavenger; and

a microcidal compound;

wherein said aqueous medium is in a liquid state.

2. The medium of claim 1, further comprising a reducing agent, a chelator, a detergent, a chaotrope, or any combination thereof.

3. The medium of claim 1, further comprising a sample comprising biomolecules.

4. The medium of claim 1, wherein said medium is inert with respect to methods of nucleic acid amplification, nucleic acid digestion, and/or protein digestion.

5. A kit for stabilizing biomolecules comprising:

a container containing the aqueous medium of claim 1, and;

instructions for using said aqueous medium for stabilization.

6. The kit of claim 5, further comprising:

a sample carrier comprising a container and a sample node, wherein said sample node is supported by said carrier and comprises the aqueous medium.

7. The kit of claim 5, further comprising a sample carrier comprising a plurality of containers, wherein each of said plurality of containers comprising the aqueous medium.

8. The kit of claim 5, further comprising a sample carrier comprising a plurality of containers wherein the plurality of containers is a multi-well plate.

9. The kit of claim 5, further comprising instructions for storing biomolecules.

10. A method of stabilizing biomolecules in aqueous solution, said method comprising:

mixing a sample comprising biomolecules with an aqueous medium in liquid state comprising boric acid or a corresponding salt of boric acid, a plasticizer and a hydroxyl radical scavenger wherein histidine is the plasticizer and the hydroxyl radical scavenger, and a microcidal compound.

11. The method of claim 10, wherein said sample is carried by a solid support.

12. The method of claim 10, wherein said sample is a solid tissue sample.

13. The method of claim 10, wherein said sample is selected from the group consisting of DNA, RNA, nucleic acids, polynucleotides, oligonucleotides, amino acids, peptides, and polypeptides.

14. The method of claim 10, wherein said mixture is maintained at about 4°

C. to about 37°

C.