Systems, methods, and workflows for optogenetics analysis
First Claim
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1. A method for measuring network effects, the method comprising:
- using an optical actuator of electrical activity incorporated into a first plurality of cells of one cell type;
obtaining a signal using an optical reporter of electrical activity incorporated into a second plurality of cells of another cell type; and
evaluating the signal.
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Abstract
The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.
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Citations
8 Claims
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1. A method for measuring network effects, the method comprising:
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using an optical actuator of electrical activity incorporated into a first plurality of cells of one cell type; obtaining a signal using an optical reporter of electrical activity incorporated into a second plurality of cells of another cell type; and evaluating the signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification