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Methods for evaluation of hemostasis

  • US 10,161,944 B2
  • Filed: 05/29/2018
  • Issued: 12/25/2018
  • Est. Priority Date: 02/15/2011
  • Status: Active Grant
First Claim
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1. A method for evaluating hemostasis in a subject, comprising:

  • providing a disposable cartridge including a fluid pathway comprising an inlet for receiving a blood sample of the subject, wherein the inlet communicates with an inlet channel in the cartridge, the inlet channel communicating with at least a first channel and a second channel, wherein the first channel is in fluid communication with a first test chamber and the second channel is in fluid communication with a second test chamber, and wherein the disposable cartridge includes a thermally conductive portion;

    introducing the blood sample into the inlet and then the inlet channel of the cartridge, wherein a first portion of the blood sample is received in the first channel and the first test chamber and a second portion of the blood sample is received in the second channel and the second test chamber;

    adjusting the temperature of the blood sample, or a portion thereof, along the thermally conductive portion;

    allowing the first portion of the blood sample to mix with a first reagent or combination of reagents to produce a first test sample;

    allowing the second portion of the blood sample to mix with a second reagent or combination of reagents to form a second test sample;

    initiating displacement within the first test sample;

    interrogating the first test sample in the first test chamber to measure a first change in mechanical properties of the first test sample;

    determining, by a processor, a first curve associated with stiffness of the first test sample as a function of time;

    determining at least one parameter including a first parameter selected from the group consisting of clot time, clot stiffness, clot formation rate, and lysis time from the first curve;

    initiating displacement within the second test sample;

    interrogating the second test sample in the second test chamber to measure a second change in mechanical properties of the second test sample;

    determining, by the processor, a second curve associated with stiffness of the first test sample as a function of time; and

    determining at least one parameter including a second parameter selected from the group consisting of clot time, clot stiffness, clot formation rate, and lysis time from the second curve;

    wherein said first parameter, said second parameter, or a combination thereof provides an indication of a state of hemostasis in the subject; and

    wherein the interrogation to determine the first parameter, the second parameter, or a combination thereof is based on a viscoelastic measurement of the blood sample.

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