Apparatus and method for developing formulations

• US 10,161,946 B2
• Filed: 08/03/2016
• Issued: 12/25/2018
• Est. Priority Date: 12/01/2008
• Status: Active Grant

• Current Assignee: Salah M. Aouad
• Original Assignee: Salah M. Aouad
• Inventors: Aouad, Salah M.
• Primary Examiner(s): Niesz, Jason K
• Application Number:
  US15/227,882
• Publication Number:
  US 20170016926A1
• Time in Patent Office: 874 Days
• CPC Class Codes: B01F 21/30 Workflow diagrams or layout of plants, e.g. flow charts; Details of workflow diagrams or layout of plants, e.g. controlling means
• CPC Class Codes: B01F 2101/06 Mixing of food ingredients
• CPC Class Codes: B01F 2101/22 Mixing of ingredients for pharmaceutical or medical compositions
• CPC Class Codes: B01F 2101/23 Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
• CPC Class Codes: B01F 33/84 Mixing plants with mixing receptacles receiving material dispensed from several component receptacles, e.g. paint tins
• CPC Class Codes: B01F 33/846 using stored recipes for determining the composition of the mixture to be produced, i.e. for determining the amounts of the basic components to be dispensed from the component receptacles
• CPC Class Codes: B01F 35/13 using one or more of the components of the mixture to wash-out the mixer
• CPC Class Codes: B01F 35/1453 by means of jets of fluid, e.g. air
• CPC Class Codes: B01F 35/2117 Weight
• CPC Class Codes: B01F 35/715 Feeding the components in several steps, e.g. successive steps
• CPC Class Codes: B01F 35/832 Flow control by weighing
• CPC Class Codes: B01F 35/88 by feeding the materials batchwise
• CPC Class Codes: B01F 35/881 by weighing, e.g. with automatic discharge
• CPC Class Codes: B01F 35/92 for heating the outside of the receptacle, e.g. heated jackets or burners
• CPC Class Codes: G01G 19/22 for apportioning materials by weighing prior to mixing them ratio regulation G05D11/00
• CPC Class Codes: G01G 19/24 using a single weighing apparatus
• CPC Class Codes: G01N 35/00584 Control arrangements for automatic analysers
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1065 : Multiple transfer devices

Abstract

The various embodiments of the present invention generally relate to an automated apparatus, wherein the automated apparatus is configured to formulate a target entity, thereby developing formulations of the target entity by using a high throughput screening (HTS) procedure and method of employing the presented automated apparatus is also disclosed herein. Other advantages that are understood from the present disclosure will further be apparent to the reader.

Claims

1. An automated system for developing new formulations of a target entity, the system comprising:

an automated apparatus comprising;

a plurality of screening lines, wherein each of the plurality of screening lines comprises;

a plurality of upstream storage containers adapted to receive raw materials, wherein the raw materials comprise the target entity and secondary ingredients;

at least one device that is adapted to condition before they are received in the storage containers, while they are in the storage containers, or after they exit the storage containers;

a dispensing system comprising a central common collector and a plurality of conduits connected to each of the storage containers;

a dosing system that measures amounts of the target entity and the secondary ingredients to be combined as an ingredient combination;

a collector that pools the target entity and the secondary ingredients as the ingredient combination;

a plurality of formulation chambers adapted to prepare formulations from the ingredient combination and perform stress treatment on the prepared formulations;

a plurality of in-line sensors installed in the formulation chambers for monitoring properties of the ingredient combination and screening formulations of the ingredient combination;

a plurality of actuators comprising pumps, valves, nozzles, and stirrers adapted to transfer raw materials and formulation between compartments and chambers;

at least one injector that is adapted to administer additional target entity and secondary ingredients into one of the formulation chambers to be formulated with the ingredient combination in that formulation chamber;

an auto-sampler for drawing out a portion of formulations prepared by the formulation chambers as samples or final formulated materials;

at least one carousel for holding a plurality of vessels for containing the drawn samples or final formulated materials; and

a waste line for evacuating waste out of the apparatus after a cleaning cycle;

a plurality of analytical devices coupled to the apparatus that are controlled by a computer; and

an articulated robotic arm connected to the apparatus and operated by the computer for automatically loading and unloading the formulation chambers and vessels between the apparatus and the analytical devices for analyses.

2. The system of claim 1, wherein the plurality of screening lines comprise 5 to 50 screening lines that are adapted to conduct high throughput screening or ultrahigh throughput screening of ingredients and formulations.

3. The system of claim 1, wherein the apparatus is adapted to handle a chemical or a biological molecule including a nucleic acid, protein, antibody, peptide, pharmaceutical, biological drug, medicine, diagnostic reagent, cosmetic, nutrient, chemical reagent, polymer, nanostructure or a combination thereof.

4. The system of claim 1, wherein the apparatus is adapted to handle a pharmaceutical, a chemical or a food ingredient including liquids, solvents, solids, vehicles, oils, buffers, solubilizers, surfactants, stabilizers, antimicrobials, preservatives, sweeteners, flavors, dyes, additives, excipients or a combination thereof.

5. The system of claim 1, wherein the apparatus is adapted to categorize the raw materials in storage containers as separate libraries, wherein one or more libraries are dedicated to target entities and multiple libraries are dedicated to secondary ingredients.

6. The system of claim 1, wherein one of the prepared formulations is cold or radioactive solution, a chemical or biological medicine, a diagnostic reagent, a cosmetic/fragrance, an agrochemical, a coating solution, a food recipe or a processed food preparation or a combination thereof.

7. The system of claim 1, wherein the conditioning of raw materials comprises degassing, heating, cooling or making a gradient of materials or a combination thereof.

8. The system of claim 1, wherein the central common collector is adapted to perform high speed parallel dispensing and the plurality of conduits are flexible conduits configured to perform high precision dispensing and dosing.

9. The system of claim 1, wherein the dosing system comprises a mechanism configured to measure weight of the target entity and secondary ingredients and a mechanism configured to measure flow rate of the target entity and secondary ingredients.

10. The system of claim 9, wherein the mechanism configured to measure weight of the target entity and secondary ingredients comprise load cells installed on the storage containers.

11. The system of claim 1, wherein the plurality of actuators further comprise heating and cooling devices, homogenizers, UV and visible light sources or a combination of thereof that are configured to process the raw materials, adjust formulations, and introduce acute stress into formulations.

12. The system of claim 1, wherein the plurality of sensors comprise a thermometer, a volume detector, a pH-meter, a nephelometer, a viscosimeter, a particle counter, a camera or a combination thereof.

13. The system of claim 1, wherein the analytical devices comprise at least a plate reader, a HPLC, a LC/MS, a NMR, a SPR/Biacore, or a combination thereof that is used to complement the monitoring and screening activities of the in-line sensors.

14. The system of claim 1, wherein the formulation chambers include single multi-FC and multi-SC plates that may be loaded or unloaded from the apparatus manually or automatically using the robotic arm for long term external incubations followed by reloading to the apparatus or for disposal and replacement by new unpacked sterile plates.

15. A method for developing new formulations of a target entity, the method comprising:

receiving the target entity to be formulated;

conditioning the received target entity;

combining the target entity with secondary ingredients to produce an ingredient combination;

formulating the ingredient combination to produce formulations;

subjecting the formulations to a stress treatment;

screening the formulations for the target entity'"'"'s physical, chemical and functional properties;

delivering a portion of the formulations as samples or final formulations to a plurality of vessels; and

analyzing and comparing screening data from screening lines for finding hit formulations.

16. The method of claim 15, wherein the preparation of ingredient combination is based on a mechanism of high speed combinatorial formulation consisting in preparing multiple separate ingredient combinations at once in a short time, through the simultaneous execution of parallel ingredient dispensing in each screening line and differential ingredient dispensing between different screening lines.

17. The method of claim 15, wherein the step of screening comprises at least three screening types, and the at least three screening types comprise formulation screening involving in-line monitoring of the target entity'"'"'s properties, stability screening involving in-line and tandem monitoring of the target entity'"'"'s alteration under stress, and functional screening involving off-line monitoring of the target entity'"'"'s functional activities.

18. The method of claim 15, wherein the stress treatment comprises chemical stress, physical stress or a combination thereof that can be executed in-line in stress chambers for short term forced stability studies and be performed outside the apparatus for long term stability studies.

19. The method of claim 15, wherein the step of analyzing and comparing comprises comparing all produced formulations utilizing a computer and a plurality of analytic devices.

20. A computer comprising a processor, a non-transient memory coupled to the processor, a set of computer instructions contained in the non-transient memory that are executable by the processor, the set of computer instructions when executed by the processor causes the processor to instruct an automated system for developing new formulations of a target entity comprising an automated apparatus, a plurality of analytic devices, and an articulated robotic arm to perform the steps of:

conditioning the target entity and secondary ingredients received in the apparatus;

dispensing the target entity and secondary ingredients through a dispensing system of the apparatus comprising a central common collector and a plurality of conduits, the target entity and secondary ingredients are dispensed to the central common collector via the plurality of conduits;

measuring amounts of the target entity and the secondary ingredient to be combined as an ingredient combination;

pooling the target entity and the secondary ingredients as the ingredient combination;

preparing formulations from the ingredient combination and performing stress treatment in a plurality of formulation chambers of the apparatus;

monitoring properties of the ingredient combination and screening formulations of the ingredient combination through a plurality of in-line sensors installed in the formulation chambers;

transferring the target entity and the secondary ingredients between compartments and chambers of the apparatus via a plurality of actuators comprising pumps, valves, nozzles, and stirrers;

administering additional target entity and secondary ingredients into one of the formulation chambers to be formulated with the ingredient combination in that formulation chamber from an injector;

drawing out a portion of formulations prepared by the formulation chambers as samples or final formulated materials to a carousel for holding a plurality of vessels for containing the drawn samples or final formulated materials;

instructing the analytical devices to screen the prepared formulations and find hit formulations; and

operating the robotic arm to load and unload the formulation chambers and vessels between the apparatus and the analytics devices for analysis.