Combinational use of mechanical manipulation and programin derivatives to increase Oct4, Sox2, or Nanog expression in fibroblasts
First Claim
Patent Images
1. A method for increasing Oct4, Sox2, or Nanog expression in fibroblasts, the method comprising:
- (a) culturing the fibroblasts in a vessel;
(b) mechanically agitating the fibroblasts that are unattached to the vessel to form a non-adherent cellular aggregate; and
(c) exposing the non-adherent cellular aggregate to an effective amount of a compound of Formula II-IX having any one of the following structures;
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides methods and compositions for inducing pluripotency in differentiated mammalian cells. In particular, the methods include mechanically aggregating the cells into discrete masses or embryoid-like bodies and treated them with a small molecule compound. Provided herein are the compositions of the compounds which are derived from programin (e.g., reversine).
-
Citations
10 Claims
-
1. A method for increasing Oct4, Sox2, or Nanog expression in fibroblasts, the method comprising:
-
(a) culturing the fibroblasts in a vessel; (b) mechanically agitating the fibroblasts that are unattached to the vessel to form a non-adherent cellular aggregate; and (c) exposing the non-adherent cellular aggregate to an effective amount of a compound of Formula II-IX having any one of the following structures; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
Specification