Compositions and methods for control of insect infestations in plants

• US 10,167,484 B2
• Filed: 04/15/2016
• Issued: 01/01/2019
• Est. Priority Date: 04/09/2004
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Baum, James A., Gilbertson, Larry A., Kovalic, David K., LaRosa, Thomas J., Lu, Maolong, Munyikwa, Tichafa R. I., Roberts, James K., Wu, Wei, Zhang, Bei
• Primary Examiner(s): Zheng, Li
• Application Number:
  US15/130,684
• Publication Number:
  US 20160237453A1
• Time in Patent Office: 991 Days
• Field of Search: 800279
• CPC Class Codes: A01N 63/23 B. thuringiensis
• CPC Class Codes: A01N 63/50 Isolated enzymes; Isolated proteins peptides A01N37/46
• CPC Class Codes: A01N 63/60 Isolated nucleic acids
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 15/8279 for biotic stress resistance, pathogen resistance, disease resistance
• CPC Class Codes: C12N 15/8285 for nematode resistance
• CPC Class Codes: C12N 15/8286 for insect resistance
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

Claims

1. A method for controlling Western Corn Rootworm infestation on a plant, comprising topically applying to the plant a pesticide composition comprising a dsRNA targeting for suppression an essential gene in the Western Corn Rootworm and providing the plant in the diet of the Western Corn Rootworm, wherein the plant comprises a nucleic acid sequence encoding at least a first Bacillus thuringiensis insecticidal protein for controlling the Western Corn Rootworm.

2. The method of claim 1, wherein the Bacillus thuringiensis insecticidal protein, or proteins, is selected from the group consisting of a Cry3, a TIC851, a CryET70, a Cry22, a binary insecticidal protein CryET33 and CryET34, a binary insecticidal protein CryET80 and CryET76, a binary insecticidal protein TIC100 and TIC101, a binary insecticidal protein PS149B1, a VIP insecticidal protein, a TIC900 or related protein, a TIC901, a TIC1201, a TIC407, a TIC417, and an insecticidal chimera thereof.

3. The method of claim 1, wherein the dsRNA is topically applied using a spray mixer, by expressing the dsRNA in a microbe and applying the microbe onto the plant, or by applying the dsRNA in a topical composition.

4. The method of claim 3, wherein the topical composition further comprises a Bacillus thuringiensis insecticidal protein.

5. The method of claim 1, wherein the composition comprises dsRNA encapsulated in a synthetic matrix and applied to the plant surface or a seed coating.

6. The method of claim 5, wherein said matrix is a polymer.

7. The method of claim 5, wherein the composition is topically applied as a microbe that is engineered to express the dsRNA, or comprises a fermentation product from the microbe.

8. The method of claim 1, wherein the dsRNA is transcribed from a DNA sequence of at least about 19 contiguous nucleotides selected from the group consisting of SEQ ID NO:

1-143, SEQ ID NO;

169-174, and the complement thereof.

9. The method of claim 1, wherein the diet is selected from the group consisting of a plant cell, a plurality of plant cells, a plant tissue, a plant root, and a plant seed, wherein the diet comprises a pest inhibitory amount of the dsRNA.