Integrated sequencing apparatuses and methods of use
First Claim
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1. A method of sequencing a nucleic acid molecule, the method comprising the steps of:
- (a) providing a droplet manipulation apparatus for performing droplet operations, the droplet manipulation apparatus comprising a substrate surface comprising an array of dynamic pads, wherein a droplet dispensed onto the substrate surface moves along a desired path defined by the dynamic pads, and wherein at least one of the pads comprises a hydrophilic patch;
(b) transporting a droplet comprising one or more nucleic acid molecules to be sequenced to the hydrophilic patch;
(c) immobilizing the one or more nucleic acid molecules;
(d) sequencing the one or more nucleic acid molecules.
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Abstract
Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.
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17 Claims
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1. A method of sequencing a nucleic acid molecule, the method comprising the steps of:
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(a) providing a droplet manipulation apparatus for performing droplet operations, the droplet manipulation apparatus comprising a substrate surface comprising an array of dynamic pads, wherein a droplet dispensed onto the substrate surface moves along a desired path defined by the dynamic pads, and wherein at least one of the pads comprises a hydrophilic patch; (b) transporting a droplet comprising one or more nucleic acid molecules to be sequenced to the hydrophilic patch; (c) immobilizing the one or more nucleic acid molecules; (d) sequencing the one or more nucleic acid molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification