Diagnostic molecular markers for seed lot purity traits in soybeans
First Claim
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1. A method of molecular marker assisted soybean breeding, the method comprising the steps of:
- a) genotyping two parental soybean plants to determine the allelic state of each parental soybean plant with regard to each of;
(i) whether the parental soybean plant comprises the W1 Locus “
DD”
allelic form (W1 allele) represented by SEQ ID NO;
1 or the W1 Locus “
II”
allelic form (w1 allele) represented by SEQ ID NO;
2;
(ii) whether the parental soybean plant comprises the T Locus “
CC”
or “
TT”
allelic form of the molecular marker represented by SEQ ID NO;
8 (M0243191);
(iii) whether the parental soybean plant comprises the Td Locus “
II”
allelic form represented by SEQ ID NO;
13 or the Td Locus “
DD”
allelic form represented by SEQ ID NO;
14;
(iv) whether the parental soybean plant comprises the R locus “
AA”
or “
TT”
allelic form of the molecular marker represented by SEQ ID NO;
19 (M0100925); and
(v) for the haplotype defined by the molecular markers represented by SEQ ID NO;
26 (M0202726), SEQ ID NO;
33 (M0119618), and SEQ ID NO;
40 (M0094170), whether the parental soybean plant comprises the L2 Locus “
AA”
or “
TT”
allelic form of the molecular marker represented by SEQ ID NO;
26 (M0202726), whether the parental soybean plant comprises the L2 Locus “
AA”
or “
TT”
allelic form of the molecular marker represented by SEQ ID NO;
33 (M0119618), and whether the parental soybean plant comprises the L2 Locus “
AA”
or “
GG”
allelic form of the molecular marker represented by SEQ ID NO;
40 (M0094170);
(b) crossing the two parental soybean plants genotyped in step (a) to generate an F1 population of soybean plants;
(c) obtaining a DNA or RNA sample from a tissue of at least one F1 soybean plant of the F1 population generated by the cross in step (b);
(d) determining by a nucleic acid based analyses, for at least the allelic forms in step (a) that differ between the two crossed parental plants, the allelic state of the F1 soybean plant; and
(e) selecting based on the determination of allelic state in step d an F1 soybean plant for use in a soybean breeding program that is a true F1 soybean plant and not the result of self-pollination of one of the parental soybean plants.
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Abstract
The present invention is in the field of plant breeding. More specifically, the invention includes a method for breeding and selecting plants that uniform for one or more seed lot purity traits such as, such as distinct flower color, pubescence color, hilum color, and pod wall color. The invention further includes molecular markers associated with distinct flower color, pubescence color, hilum color, and pod wall color for uses in a breeding program.
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4 Claims
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1. A method of molecular marker assisted soybean breeding, the method comprising the steps of:
-
a) genotyping two parental soybean plants to determine the allelic state of each parental soybean plant with regard to each of; (i) whether the parental soybean plant comprises the W1 Locus “
DD”
allelic form (W1 allele) represented by SEQ ID NO;
1 or the W1 Locus “
II”
allelic form (w1 allele) represented by SEQ ID NO;
2;(ii) whether the parental soybean plant comprises the T Locus “
CC”
or “
TT”
allelic form of the molecular marker represented by SEQ ID NO;
8 (M0243191);(iii) whether the parental soybean plant comprises the Td Locus “
II”
allelic form represented by SEQ ID NO;
13 or the Td Locus “
DD”
allelic form represented by SEQ ID NO;
14;(iv) whether the parental soybean plant comprises the R locus “
AA”
or “
TT”
allelic form of the molecular marker represented by SEQ ID NO;
19 (M0100925); and(v) for the haplotype defined by the molecular markers represented by SEQ ID NO;
26 (M0202726), SEQ ID NO;
33 (M0119618), and SEQ ID NO;
40 (M0094170), whether the parental soybean plant comprises the L2 Locus “
AA”
or “
TT”
allelic form of the molecular marker represented by SEQ ID NO;
26 (M0202726), whether the parental soybean plant comprises the L2 Locus “
AA”
or “
TT”
allelic form of the molecular marker represented by SEQ ID NO;
33 (M0119618), and whether the parental soybean plant comprises the L2 Locus “
AA”
or “
GG”
allelic form of the molecular marker represented by SEQ ID NO;
40 (M0094170);(b) crossing the two parental soybean plants genotyped in step (a) to generate an F1 population of soybean plants; (c) obtaining a DNA or RNA sample from a tissue of at least one F1 soybean plant of the F1 population generated by the cross in step (b); (d) determining by a nucleic acid based analyses, for at least the allelic forms in step (a) that differ between the two crossed parental plants, the allelic state of the F1 soybean plant; and (e) selecting based on the determination of allelic state in step d an F1 soybean plant for use in a soybean breeding program that is a true F1 soybean plant and not the result of self-pollination of one of the parental soybean plants. - View Dependent Claims (2, 3, 4)
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Specification