Microplate and methods for making the same

• US 10,173,218 B2
• Filed: 07/15/2009
• Issued: 01/08/2019
• Est. Priority Date: 07/17/2008
• Status: Active Grant

• Current Assignee: LGC Genomics Ltd.
• Original Assignee: Douglas Scientific LLC
• Inventors: Peterson, Bruce
• Primary Examiner(s): Levkovich, Natalia
• Application Number:
  US13/003,779
• Publication Number:
  US 20110123415A1
• Time in Patent Office: 3,464 Days
• Field of Search: 422554, 422553, 422552, 422551, 156510, 156506, 156515, 156517
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B29C 51/00 Shaping by thermoforming , i.e. shaping sheets or sheet like preforms after heating, e.g. shaping sheets in matched moulds or by deep-drawing; Apparatus therefor blow moulding of tubular preforms B29C49/00, deforming of tubular or hollow preforms B29C67/0014
• CPC Class Codes: B29C 65/18 using heated tools
• CPC Class Codes: B29C 66/112 Single lapped joints
• CPC Class Codes: B29C 66/131 Single flanged joints, i.e. one of the parts to be joined being rigid and flanged in the joint area
• CPC Class Codes: B29C 66/5346 said single elements being substantially flat
• CPC Class Codes: B29C 66/61 Joining from or joining on the inside for making tubes by bending sheets and joining from the inside B29C53/387
• CPC Class Codes: B29L 2031/712 Containers; Packaging elements or accessories, Packages closures therefor B29L2031/56; ink or toner cartridges B29L2031/7678; squeeze tubes B29L2023/20; suitcases B29L2031/7418
• CPC Class Codes: Y10T 156/1062 Prior to assembly

Abstract

A microplate (10) includes a carrier (12) having a plate (20) and an annular perimeter wall (30) to define a recess (34). An array of holes (26) extends through the plate (20). A tape piece (16), die cut from a flexible tape (60) includes an array of wells (54) each extending through and having an opening (56) extending into the well (54). The array of wells (54) has a number and locations corresponding to the array of holes (26). The openings (56) have sizes corresponding to the holes (26). An upper surface (50) of the tape piece (16) is abutted with and bonded to the bottom face (24) of the plate (20) with the openings (56) corresponding to the array of holes (26). The slideable receipt of an annular outer periphery (58) of the tape piece (16) insures that the array of wells (54) are aligned to correspond to the array of holes (26) as die cutting of tape piece (16) insures that the array of wells (54) are at consistent positions relative to the annular outer periphery (58).

Claims

1. A method for making a microplate comprising:

providing a carrier including a plate with a top face, a bottom face spaced from the top face in a direction, and an annular perimeter, with the plate including an array of through-holes extending from the top face through the bottom face and located inside the annular perimeter, with the array of holes terminating at the top face and at the bottom face, with the bottom face being substantially planar;

providing a flexible tape piece made of a single piece of material, with the flexible tape

piece including an upper surface and a lower surface spaced a thickness from the upper surface in the direction, with the flexible tape piece further including an array of closed wells, the closed wells configured and arranged to receive and store assay samples, and wherein each well

is integrally extending in the direction away from the upper and lower surfaces and each having an open end comprising an opening extending from the upper surface into the well and a closed end comprising an integral bottom spaced from the open end, with the openings of the array of wells having sizes generally corresponding to the array of through-holes of the plate, with at least a portion of the array of wells having a number and locations generally corresponding to the array of holes of the plate, with the carrier formed of material more rigid than the material forming the flexible tape piece; and

abutting and bonding at least a portion of the upper surface of the tape with at least a portion of the bottom face of the plate so that at least a portion of the openings of the array of wells generally correspond to the array of holes of the plate.

2. The method as claimed in claim 1, wherein the carrier has a recess including the bottom face formed therein, with providing the flexible tape piece including providing the flexible tape piece having an annular outer periphery extending between and substantially perpendicular to the upper and lower surfaces, and with abutting and bonding the upper surface with the plate including moving the annular outer periphery of the flexible tape piece into the recess of the carrier.

3. The method as claimed in claim 2, wherein the carrier has the recess with a size corresponding to and for slideably receiving the annular outer periphery of the flexible tape piece, and with abutting and bonding the upper surface with the plate including aligning the annular outer periphery of the flexible tape piece with the recess and sliding the annular outer periphery of the flexible tape piece in the recess to at least partially align at least a portion of the openings of the array of wells with the array of holes of the plate.

4. The method as claimed in claim 3, wherein the carrier includes an annular perimeter wall extending from the plate and extending in a direction substantially perpendicular to the top and bottom faces, with the annular perimeter wall including an annular inner face, with the recess defined by the bottom face and the annular inner face, and with sliding the annular outer periphery of the flexible tape piece into the recess including abutting the annular outer periphery of the flexible tape piece with the annular inner face of the annular perimeter wall of the carrier and moving the flexible tape piece in the direction toward the bottom face of the plate.

5. The method as claimed in claim 4, wherein the carrier with the annular perimeter wall including a lower edge has a perimeter and a spacing to the top face larger than to the bottom face, with the carrier further including a lip extending along the perimeter of the lower edge in the direction away from the top face, with the lip including a horizontal section parallel to and spaced from the top and bottom faces in the direction, with the lip further including a vertical section perpendicular to and spaced from the top and bottom faces in the direction, with the vertical section being spaced from the top and bottom faces in a horizontal direction perpendicular to the direction.

6. The method as claimed in claim 5, with providing the flexible tape piece comprising feeding a flexible tape with a plurality of tractor feed holes formed along two parallel, spaced, longitudinal edges of the flexible tape, with the flexible tape including a plurality of arrays of wells spaced from one another along a longitudinal axis of the flexible tape perpendicular to the direction and intermediate the plurality of tractor feed holes formed along the two parallel, spaced, longitudinal edges, and with providing the flexible tape piece further comprising die cutting the flexible tape by moving a cutting die in a direction perpendicular to the upper and lower surfaces and against the flexible tape located upon an anvil.

7. The method as claimed in claim 6, with feeding the flexible tape including feeding the flexible tape having the thickness between the upper and lower surfaces in the direction and providing flexibility to allow rolling, with providing the carrier including providing the plate having a thickness between the top and bottom faces in the direction and multiple times the thickness of the flexible tape piece sufficient to resist flexing.

8. The method as claimed in claim 6, with the abutting and bonding including bonding selected from the group consisting of a bonding by a two-sided tape, thermo-bonding and bonding by adhesive.

9. The method as claimed in claim 6, with the abutting and bonding including placing the plate and the flexible tape piece between a top pressing jaw and a bottom pressing jaw spaced from the top pressing jaw in the direction, with the bottom pressing jaw including an array of apertures having a number and locations corresponding to the array of wells, with the abutting and bonding further including moving the top pressing jaw relative to the bottom pressing jaw in the direction to sandwich at least a portion of the upper surface to the bottom face of the plate with the openings of the array of wells at least partially aligned with the array of holes of the plate and with the array of wells at least partially corresponding to the array of apertures of the bottom pressing jaw.

10. The method as claimed in claim 1, with the abutting and bonding including placing the plate and the flexible tape piece between a top pressing jaw and a bottom pressing jaw spaced from the top pressing jaw in the direction, with the bottom pressing jaw including an array of apertures having a number and locations generally corresponding to the array of wells, with the abutting and bonding further including moving the top pressing jaw relative to the bottom pressing jaw in the direction to sandwich at least a portion of the upper surface to the bottom face of the plate with the openings of the array of wells at least partially aligned with the array of holes of the plate and with the array of wells at least partially corresponding to the array of apertures of the bottom pressing jaw.

12. The method as claimed in claim 1, with providing the carrier comprising providing the carrier plate with each of the array of holes extending from a top face to a bottom face of the carrier plate, wherein each of the holes are a size larger than the openings of the wells at the upper surface of the array of wells.

13. The method of claim 1, wherein the flexible tape piece includes a plurality of tractor feed holes.

14. The method of claim 1, wherein the closed wells comprise a clear and flat bottom suitable for assays requiring optical readout.

11. A method for making a microplate comprising:

providing a carrier including a plate with a top face, a bottom face spaced from the top face in a direction, and an annular perimeter, with the plate including an array of through-holes extending from the top face through the bottom face and located inside the annular perimeter, with the array of holes terminating at the top face and at the bottom face, with the bottom face being substantially planar;

cutting a tape piece as a single piece including an upper surface and a lower surface spaced a thickness from the upper surface in the direction, with the tape piece as cut further including an array of wells, the wells being closed wells that are configured and arranged to each receive samples and/or reagents and to store samples, and wherein the wells each integrally

extend in the direction away from the upper and lower surfaces and each having an open end comprising an opening extending from the upper surface into the well and a closed end comprising an integral bottom spaced from the opening, with the openings of the array of wells having sizes generally corresponding to the array of holes of the plate, with the array of wells having a number and locations generally corresponding to the array of holes of the plate, with the carrier formed of material more rigid than material forming the single piece; and

abutting and bonding at least a portion of the upper surface to at least a portion of the bottom face of the plate with the openings of the array of wells generally corresponding to the array of holes of the plate.

15. The method as claimed in claim 11, with cutting the tape piece comprising feeding a flexible tape with a plurality of tractor feed holes formed along two parallel, spaced, longitudinal edges of the flexible tape, with the flexible tape including a plurality of arrays of wells spaced from one another along a longitudinal axis of the flexible tape perpendicular to the direction and intermediate the plurality of tractor feed holes formed along the two parallel, spaced, longitudinal edges, with cutting the tape piece further comprising die cutting the flexible tape by moving a cutting die in a direction, perpendicular to the upper and lower surfaces and against the flexible tape located upon an anvil.

16. The method as claimed in claim 15, with feeding the flexible tape including feeding the flexible tape having the thickness between the upper and lower surfaces in the direction and providing flexibility to allow rolling, and with providing the carrier including providing the plate having a thickness between the top and bottom faces in the direction and multiple times the thickness of the flexible tape sufficient to resist flexing.

17. The method as claimed in claim 16, with the abutting and bonding including bonding selected from the group consisting of bonding by a two-sided tape, thermo-bonding and bonding by adhesive.

18. The method of claim 11, wherein the tape is a flexible tape.

19. The method of claim 18, wherein the flexible tape includes a plurality of tractor feed holes.