Methods and compositions for incorporating nucleotides
First Claim
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1. A labeled reversible terminator comprising a nucleotide analogue of dATP having a 3′
- -O group and a label, wherein the label is ROX and is attached to the nucleotide analogue of dATP though a cleavable linker comprising a disulfide bond, and wherein a removable chemical moiety caps the 3′
-O group, wherein said labeled reversible terminator is in a mixture in solution with an unlabeled reversible terminator comprising a nucleotide analogue of dATP, wherein the concentration of the unlabeled nucleotide analogue of dATP is present in said solution at a higher concentration than the labeled nucleotide analogue of dATP.
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Abstract
The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
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4 Claims
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1. A labeled reversible terminator comprising a nucleotide analogue of dATP having a 3′
- -O group and a label, wherein the label is ROX and is attached to the nucleotide analogue of dATP though a cleavable linker comprising a disulfide bond, and wherein a removable chemical moiety caps the 3′
-O group, wherein said labeled reversible terminator is in a mixture in solution with an unlabeled reversible terminator comprising a nucleotide analogue of dATP, wherein the concentration of the unlabeled nucleotide analogue of dATP is present in said solution at a higher concentration than the labeled nucleotide analogue of dATP. - View Dependent Claims (2, 3, 4)
- -O group and a label, wherein the label is ROX and is attached to the nucleotide analogue of dATP though a cleavable linker comprising a disulfide bond, and wherein a removable chemical moiety caps the 3′
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