Methods for nucleic acid sequencing
First Claim
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1. A method of identifying a single molecule in a sample comprising two or more types of labeled molecules, the method comprising:
- determining a luminescent lifetime and a luminescent intensity of the single molecule in the sample; and
identifying the single molecule as one of the two or more types of labeled molecules based on a combination of both the luminescent lifetime and the luminescent intensity of the single molecule.
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Abstract
Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.
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19 Claims
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1. A method of identifying a single molecule in a sample comprising two or more types of labeled molecules, the method comprising:
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determining a luminescent lifetime and a luminescent intensity of the single molecule in the sample; and identifying the single molecule as one of the two or more types of labeled molecules based on a combination of both the luminescent lifetime and the luminescent intensity of the single molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification