Methods and compositions for increased safety of stem cell-derived populations
First Claim
1. A method for reducing residual pluripotent stem cells in an in vitro differentiated cell population, comprisingculturing an in vitro differentiated cell population at an oxygen partial pressure that is less than 142 mmHg for at least 1 day and less than 10 days, andharvesting the differentiated cell population after the culturing step;
- wherein the harvested cell population comprises less than 1 pluripotent stem cell per 103 cells,wherein 50% or more of the cells in the in vitro differentiated cell population are positive for at least one differentiation marker before the culturing step.
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Abstract
The invention provides methods and compositions relating to differentiated cell populations that derive from pluripotent stem cells. The methods relate to reducing the number of residual stem cells present in such populations. The compositions include differentiated cell populations that contain reduced number of stem cells or that contain no stem cells. Pluripotent stem cells may be reduced in number and/or function through exposure to low oxygen levels.
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Citations
17 Claims
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1. A method for reducing residual pluripotent stem cells in an in vitro differentiated cell population, comprising
culturing an in vitro differentiated cell population at an oxygen partial pressure that is less than 142 mmHg for at least 1 day and less than 10 days, and harvesting the differentiated cell population after the culturing step; -
wherein the harvested cell population comprises less than 1 pluripotent stem cell per 103 cells, wherein 50% or more of the cells in the in vitro differentiated cell population are positive for at least one differentiation marker before the culturing step. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for reducing residual pluripotent stem cells in an in vitro differentiated cell population, comprising
culturing an in vitro differentiated cell population at an oxygen partial pressure that is less than 142 mmHg for at least 1 day and less than 10 days to reduce the pluripotent stem cell number to less than 1 pluripotent stem cell per 105 cells, and harvesting the differentiated cell population after the culturing step.
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16. A method for reducing residual pluripotent stem cells in an in vitro differentiated cell population, comprising
differentiating a population of pluripotent stem cells into an in vitro differentiated cell population under normoxic conditions, culturing the in vitro differentiated cell population at an oxygen partial pressure that is less than 142 mmHg for at least 1 day and less than 10 days, and harvesting the differentiated cell population after the culturing step; wherein the harvested cell population comprises less than 1 pluripotent stem cell per 103 cells. - View Dependent Claims (17)
Specification